Application
Western blot
Sample
Rat Cell lysate - whole cell (rat Bone marrow derived macrophages)
Loading amount
15 µg
Specification
rat Bone marrow derived macrophages
Treatment
stimulants of macrophage activation for 4 hours
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBSt milk
Secondary antibody
Name
Abcam antibody:Rabbit polyclonal Secondary Antibody to Goat IgG - H&L (HRP)
Dilution
1/10000
Detection
Detection method
ecl advance
Exposure
30 second(s)
Bands
Specific: 30 kDa
Additional data
Additional Notes
lane 1 rat bone marrow macrophages
lane 2 rat bone marrow macrophages + IL4
lane 3 rat bone marrow macrophages + IFN
lane 2 rat bone marrow macrophages + IL4
lane 3 rat bone marrow macrophages + IFN
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Apr 04 2008