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    socs1-antibody-ab9870.pdf

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Signal Transduction Adapters Cytoplasmic
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Anti-SOCS1 抗体 (ab9870)

  • Datasheet
Reviews (4)Q&A (4)References (25)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Flow Cytometry (Intracellular) - Anti-SOCS1 antibody (ab9870)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (ab9870)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (ab9870)
  • Immunocytochemistry - Anti-SOCS1 antibody (ab9870)

Key features and details

  • Goat polyclonal to SOCS1
  • Suitable for: Flow Cyt (Intra), ICC/IF, ICC, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human, Pig
  • Isotype: IgG

こちらの製品もご検討ください

アイソタイプ
FITC Mouse IgG1 kappa [MOPC-21] - Isotype Control (ab106163)
二次抗体
Product image
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
一次抗体
Product image
Alexa Fluor® 405 Anti-Calreticulin antibody [EPR3924] - ER Marker (ab210431)

関連製品

製品の概要

  • 製品名

    Anti-SOCS1 antibody
    SOCS1 一次抗体 製品一覧
  • 製品の詳細

    Goat polyclonal to SOCS1
  • 由来種

    Goat
  • アプリケーション

    適用あり: Flow Cyt (Intra), ICC/IF, ICC, Flow Cyt, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Pig
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide corresponding to Human SOCS1 aa 150-250 (C terminal).
    Database link: NP_003736.1

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • Flow Cyt (Intra): HEK293 cells.
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    pH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 0.5% Tris, 0.5% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Signal Transduction
    • Adapters
    • Cytoplasmic
    • Immunology
    • Immune System Diseases
    • Autoimmune
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • STATs
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus

関連製品

  • Compatible Secondaries

    • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
    • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
  • Isotype control

    • Goat IgG, polyclonal - Isotype Control (ab37373)
  • Positive Controls

    • Mouse spleen tissue lysate - total protein (ab7937)
  • Recombinant Protein

    • Recombinant Human SOCS1 protein (His tag) (ab226430)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab9870の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.
ICC/IF
Use at an assay dependent concentration. PubMed: 21628332
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 23298838

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
Flow Cyt (Intra)
Use a concentration of 10 µg/ml.
ICC/IF
Use at an assay dependent concentration. PubMed: 21628332
ICC
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration. PubMed: 23298838

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC-P
Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
  • 組織特異性

    Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
  • パスウェイ

    Protein modification; protein ubiquitination.
  • 配列類似性

    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • ドメイン

    The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
  • Target information above from: UniProt accession O15524 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8651 Human
    • Entrez Gene: 12703 Mouse
    • Entrez Gene: 252971 Rat
    • Omim: 603597 Human
    • SwissProt: O15524 Human
    • SwissProt: O35716 Mouse
    • SwissProt: Q9QX78 Rat
    • Unigene: 50640 Human
    • Unigene: 130 Mouse
    • Unigene: 82754 Rat
    see all
  • 別名

    • CIS1 antibody
    • CISH 1 antibody
    • CISH1 antibody
    • Cytokine inducible SH2 protein 1 antibody
    • JAB antibody
    • JAK binding protein antibody
    • JAK-binding protein antibody
    • Janus kinase binding protein antibody
    • SOCS 1 antibody
    • SOCS-1 antibody
    • Socs1 antibody
    • SOCS1_HUMAN antibody
    • SSI 1 antibody
    • SSI-1 antibody
    • SSI1 antibody
    • STAT induced STAT inhibitor 1 antibody
    • STAT-induced STAT inhibitor 1 antibody
    • Suppressor of cytokine signaling 1 antibody
    • Supressor of cytokine signalling 1 antibody
    • TEC interacting protein 3 antibody
    • Tec-interacting protein 3 antibody
    • TIP 3 antibody
    • TIP-3 antibody
    • TIP3 antibody
    see all

画像

  • Flow Cytometry (Intracellular) - Anti-SOCS1 antibody (ab9870)
    Flow Cytometry (Intracellular) - Anti-SOCS1 antibody (ab9870)

    Flow cytometric analysis of HEK293 cells with ab9870 (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (ab9870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (ab9870)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of paraffin embedded Human Kidney with ab9870 (8 µg/ml). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (ab9870)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOCS1 antibody (ab9870)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of paraffin embedded Mouse Kidney with ab9870 (8 µg/ml). Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

  • Immunocytochemistry - Anti-SOCS1 antibody (ab9870)
    Immunocytochemistry - Anti-SOCS1 antibody (ab9870)

    Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation with ab9870 (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (25)

ab9870 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab9870 は 25 報の論文で使用されています。

  • Lin L  et al. SPTBN1 inhibits inflammatory responses and hepatocarcinogenesis via the stabilization of SOCS1 and downregulation of p65 in hepatocellular carcinoma. Theranostics 11:4232-4250 (2021). PubMed: 33754058
  • Liu H  et al. Increased expression of IFN-? in preeclampsia impairs human trophoblast invasion via a SOCS1/JAK/STAT1 feedback loop. Exp Ther Med 21:112 (2021). PubMed: 33335575
  • Liu D  et al. microRNA-155 Modulates Hepatic Stellate Cell Proliferation, Apoptosis, and Cell Cycle Progression in Rats With Alcoholic Hepatitis via the MAPK Signaling Pathway Through Targeting SOCS1. Front Pharmacol 11:270 (2020). PubMed: 32317960
  • Warren M  et al. Association of a de novo nonsense mutation of the TRIM8 gene with childhood-onset focal segmental glomerulosclerosis. Pediatr Nephrol 35:1129-1132 (2020). PubMed: 32193649
  • Gao H  et al. MicroRNA-93 contributes to the suppression of lung inflammatory responses in LPS-induced acute lung injury in mice via the TLR4/MyD88/NF-?B signaling pathway. Int J Mol Med 46:561-570 (2020). PubMed: 32468034
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-8 of 8 Abreviews or Q&A

Western blot abreview for Anti-SOCS1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle (lane 1) and spleen (lane 2))
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
45 µg
Specification
Muscle (lane 1) and spleen (lane 2)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Dec 22 2017

Western blot abreview for Anti-SOCS1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rabbit Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
35 µg
Specification
Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Nov 30 2017

Western blot abreview for Anti-SOCS1 antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (monocyte-derived macrophage)
Loading amount
30 µg
Specification
monocyte-derived macrophage
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Jan 17 2013

Western blot abreview for Anti-SOCS1 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (rat Bone marrow derived macrophages)
Loading amount
15 µg
Specification
rat Bone marrow derived macrophages
Treatment
stimulants of macrophage activation for 4 hours
Gel Running Conditions
Reduced Denaturing (12% gel)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

投稿 Apr 04 2008

Question

Product code: 3691
Inquiry: Hi I am looking for the antibodies which could be used for human SOCS1 detection by FACS and IHC. I see that you have the facs antibody for SOCS1, could you please send me the protocol and also the optimized concentration? Thank you very much! And also I saw you have a lot of the antibodies for IHC staining for SOCS1. I would like to check the human SOCS1 expression in different organs of humanized mice. Could you give a recommendation? Thank you very much! Best regards

Read More

Abcam community

Verified customer

Asked on Sep 25 2012

Answer

Thank you for contacting us.

To our knowledge, customised protocols are not required for this product, any general Flow Cytometry protocol could be used with this antibody.

https://www.abcam.com/index.html?pageconfig=resource&rid=11380

https://www.abcam.com/index.html?pageconfig=resource&rid=11448

https://www.abcam.com/index.html?pageconfig=resource&rid=11381

https://www.abcam.com/index.html?pageconfig=popular_protocols

The optimal dilutions should be determined by end user.I wouldsuggest starting anywhere from1ug-10ug/ml.

All of these antibodies are tested with human samples and in IHC-P e.g. ab62584, ab9870, ab135718, ab83493, ab65989 so these would work. Choose any one of these for best results. Please note,because you are using mouse for tissue grafts so avoiding mouse monoclonal would be a good idea else we can suggest some extra blocking steps.

The concentration of antibody for IHC-P can be tried somewhere between 1-10ug/ml.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

Answered on Sep 25 2012

Question

Please could you tell me what differences are between these two antibodies: SOCS1 antibody (ab9870) and SOCS1 antibody (ab3691)? Which one is better to use in lysates made of human melanoma cells? Do I have to use as a secondary antibody a goat polyclonal in case of Ab9870? I need your advice to choose the best antibodies against STAT2 and SOCS2 proteins. In case of STAT2, I need to detect both phosphoforms of STAT2 (anti-phosphoSerine and anti-phosphoTyrosine specific STAT2 antibodies) as well as a whole anti-STAT2 antibody as a loading control after membranes reblot with phospho-specific STAT2 antibodies. Thank you very much in advance.

Read More

Abcam community

Verified customer

Asked on Mar 20 2006

Answer

Thank you for your enquiry. The differences between goat polyclonal to SOCS1 (ab9870) and rabbit polyclonal to SOCS1 (ab3691) is that ab9870 reacts with mouse and is predicted to react with human due to sequence homology. So far it has only been shown to work by western blotting. ab3691 reacts with human and has been shown to work by western blotting, immunoprecipitation and ELISA. We do not have details as to the best one of the two for application to human melanoma cells. Yes, you will need an anti-goat IgG secondary antibody for use with SOCS1 antibody (ab9870). For a detailed list of compatible secondaries may I suggest that you visit the datasheet of the antibody and scroll to the bottom. Unfortunately we do not sell any antibodies that are specific for the phosphorylation status of STAT2. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 21 2006

Question

It states that ab5923 detects a band of 47kD in WB when the predicted MW of SOCS1 is 24 kDa. Ab3691, also a SOCS 1 antibody, detects a band at 24 kDa - why? I still have not heard a response to the enquiry reference CRM372981 and it has been a week. This has slowed down my research and questioned everything completed thus far because it is not certain whether the antibody (ab5923) actually recognizes SOCS-1. I would like to try another SOCS-1 antibody , however, I am hesitant because I am not pleased with ab5923 and I would like to know that the antibody I am interested in, ab9870, definitely recognizes SOCS-1. Please let me know if you have any information.

Read More

Abcam community

Verified customer

Asked on Oct 12 2004

Answer

Thank you for your patience. As ab5923 was originated outside Abcam I had to contact the originator regarding your question and have just heard back. There was a mistake on the datasheet for ab5923 - it is a CIS1 antibody, not a SOCS1 antibody. I apologize for this mistake and the datasheet will be updated to reflect this information. I can of course offer you a refund or an alternative antibody free of charge. Ab9870 is a SOCS1 antibody, as well as ab3691. Please let me know what you would like.

Read More

Abcam Scientific Support

Answered on Oct 13 2004

Question

Customer is having problems with these two antibodies in WB. Secondary is working fine in other blots. Using mouse lysate. For both, has tried dilutions at 1:500, 1:1000, 1:2000. Is getting extremely high background. ab9870: lot#11815 Is seeing lots of bands of equal intensity to the expected band at approx. 30 kDa. ab3693: lot#30036 In the region of interest (27 kDa) is seeing 3 bands and other background bands of equal intensity to those.

Read More

Abcam community

Verified customer

Asked on Dec 11 2003

Answer

I'm sorry to hear that you are having problems with these two antibodies. I am enquiring with the originators of these two antibodies for suggestions that will help you out. In the meantime, below is a general protocol for ab9870 that will be helpful for you. Some suggestions: high background is usually a sign of a blocking problem. I know that you mentioned that you have tried different blocks, but the secondary may be reacting with the blocking reagent. I suggest that you test this by omiting the primary antibody and testing with only the secondary. If background appears, change either the secondary or the blocking reagent. Also, please try using the secondary at a lower concnetration. Protocol for ab9870: After transfer on PVDF membrane, membrane is blocked in 2.5% skimmed milk in TBS-T for 1 hr at room temperature with agitation (can be blocked overnight at 4C without agitation). We find enhancement of transfer of proteins on PVDF membranes compared to nitrocellulose membranes. Primary antibody (~2ug/ml) was incubated for 1 hr at room temperature with agitation. Then membrane was incubated with the secondary antibody for 1 hr at room temperature with agitation. Washing with TBST three times after primary and secondary antibody, each wash is about for 5-10 mins. We use ECL-plus (Amersham) rather than ECL, which is more sensitive for detection.

Read More

Abcam Scientific Support

Answered on Dec 15 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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