Application
Western blot
Sample
Mouse Cell lysate - whole cell (macrophage)
Loading amount
20 µg
Specification
macrophage
Treatment
IFNgamma
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS-T+5% milk
Secondary antibody
Name
Non-Abcam antibody was used: rabbit anti-goat HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
rcl
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 50 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 01 2012