Anti-SNRPA1 抗体 [EPR7557] (ab128937)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7557] to SNRPA1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SNRPA1 antibody [EPR7557]
SNRPA1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7557] to SNRPA1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Human colon and squamous lung carcinoma tissues; HeLa, HepG2, Huvec cell lysates; rat and mouse liver lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
解離定数(KD 値)
KD = 7.60 x 10 -11 M Learn more about KD -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7557 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab128937の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
This protein is associated with sn-RNP U2. It helps the A' protein to bind stem loop IV of U2 snRNA. -
配列類似性
Belongs to the U2 small nuclear ribonucleoprotein A family.
Contains 4 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6627 Human
- Entrez Gene: 68981 Mouse
- Entrez Gene: 100361269 Rat
- Omim: 603521 Human
- SwissProt: P09661 Human
- SwissProt: P57784 Mouse
- Unigene: 528763 Human
- Unigene: 22362 Mouse
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別名
- Lea1 antibody
- RU2A_HUMAN antibody
- Small nuclear ribonucleoprotein polypeptide A antibody
see all
画像
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All lanes : Anti-SNRPA1 antibody [EPR7557] (ab128937) at 1/1000 dilution
Lane 1 : Huvec cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Rat liver tissue lysate
Lane 5 : Mouse liver tissue lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa -
ab128937, at a 1/100 dilution, staining SNRPA1 in paraffin-embedded Human colon tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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ab128937, at a 1/100 dilution, staining SNRPA1 in paraffin-embedded Human squamous lung cell carcinoma tissue by immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab128937 は 4 報の論文で使用されています。
- Obuća M et al. Retinitis pigmentosa-linked mutation in DHX38 modulates its splicing activity. PLoS One 17:e0265742 (2022). PubMed: 35385551
- Yuan P et al. Identification of RNA-binding protein SNRPA1 for prognosis in prostate cancer. Aging (Albany NY) 13:2895-2911 (2021). PubMed: 33460399
- Fu K et al. Biological and RNA regulatory function of MOV10 in mammalian germ cells. BMC Biol 17:39 (2019). PubMed: 31088452
- Soboleva TA et al. A new link between transcriptional initiation and pre-mRNA splicing: The RNA binding histone variant H2A.B. PLoS Genet 13:e1006633 (2017). ChIP ; Mouse . PubMed: 28234895