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    snap25-antibody-ab5666.pdf

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Neuroscience Neurotransmission Secretory Vesicles SNAPs & SNAREs
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Anti-SNAP25 抗体 (ab5666)

  • Datasheet
  • SDS
Reviews (4)Q&A (2)References (37)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-SNAP25 antibody (ab5666)
  • Immunocytochemistry - Anti-SNAP25 antibody (ab5666)
  • Flow Cytometry - Anti-SNAP25 antibody (ab5666)
  • Immunocytochemistry - Anti-SNAP25 antibody (ab5666)
  • Western blot - Anti-SNAP25 antibody (ab5666)

Key features and details

  • Rabbit polyclonal to SNAP25
  • Suitable for: ICC, WB, Flow Cyt
  • Reacts with: Mouse, Rat, Chicken, Guinea pig, Cow, Zebrafish
  • Isotype: IgG

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
タンパク質
Product image
Recombinant Human SNAP25 protein (ab74529)

関連製品

製品の概要

  • 製品名

    Anti-SNAP25 antibody
    SNAP25 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to SNAP25
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC, WB, Flow Cytmore details
  • 種交差性

    交差種: Mouse, Rat, Chicken, Guinea pig, Cow, Zebrafish
    交差が予測される動物種: Human, Chimpanzee, Rhesus monkey, Goldfish
  • 免疫原

    Synthetic peptide corresponding to Mouse SNAP25 aa 195-206.
    Sequence:

    ANQRATKMLGSG


    (Peptide available as ab5843)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human SNAP25 protein (ab74529)
  • Recombinant Protein

    • Recombinant Human SNAP25 protein (ab74529)

アプリケーション

Our Abpromise guarantee covers the use of ab5666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 25 kDa.
Flow Cyt Use 3-5µg for 106 cells.

ターゲット情報

  • 機能

    t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
  • 組織特異性

    Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
  • 配列類似性

    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • 翻訳後修飾

    Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
  • 細胞内局在

    Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
  • Target information above from: UniProt accession P60880 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 396444 Chicken
    • Entrez Gene: 458096 Chimpanzee
    • Entrez Gene: 540853 Cow
    • Entrez Gene: 6616 Human
    • Entrez Gene: 20614 Mouse
    • Entrez Gene: 25012 Rat
    • Entrez Gene: 574204 Rhesus monkey
    • Entrez Gene: 30712 Zebrafish
    • Omim: 600322 Human
    • SwissProt: P60878 Chicken
    • SwissProt: Q5R1X1 Chimpanzee
    • SwissProt: Q17QQ3 Cow
    • SwissProt: P36978 Goldfish
    • SwissProt: P60880 Human
    • SwissProt: P60879 Mouse
    • SwissProt: P60881 Rat
    • SwissProt: P60877 Rhesus monkey
    • SwissProt: Q5TZ66 Zebrafish
    • SwissProt: Q6PC54 Zebrafish
    • Unigene: 167317 Human
    • Unigene: 45953 Mouse
    • Unigene: 107689 Rat
    see all
  • 別名

    • bA416N4.2 antibody
    • Bdr antibody
    • CMS18 antibody
    • dJ1068F16.2 antibody
    • FLJ23079 antibody
    • HGNC:11132 antibody
    • MGC105414 antibody
    • MGC139754 antibody
    • Resistance to inhibitors of cholinesterase 4 homolog antibody
    • RIC 4 antibody
    • RIC-4 antibody
    • RIC4 antibody
    • SEC 9 antibody
    • SEC9 antibody
    • SNAP 25 antibody
    • SNAP antibody
    • SNAP-25 antibody
    • SNAP-25B antibody
    • SNAP25 antibody
    • SNP 25 antibody
    • SNP25 antibody
    • SNP25_HUMAN antibody
    • sp antibody
    • SUP antibody
    • Super protein antibody
    • Synaptosomal associated 25 kDa protein antibody
    • Synaptosomal associated protein antibody
    • Synaptosomal associated protein 25 antibody
    • Synaptosomal associated protein 25kDa antibody
    • Synaptosomal-associated 25 kDa protein antibody
    • Synaptosomal-associated protein 25 antibody
    • Synaptosomal-associated protein antibody
    • Synaptosomal-associated protein, 25-KD antibody
    see all

画像

  • Western blot - Anti-SNAP25 antibody (ab5666)
    Western blot - Anti-SNAP25 antibody (ab5666)
    All lanes : Anti-SNAP25 antibody (ab5666)

    Lane 1 : Mouse cerebellum
    Lane 2 : Mouse brain
    Lane 3 : Rat brain
    Lane 4 : Membrane enriched extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate
  • Immunocytochemistry - Anti-SNAP25 antibody (ab5666)
    Immunocytochemistry - Anti-SNAP25 antibody (ab5666)
    ICC/IF image of ab5666 stained PC12 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5666, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Flow Cytometry - Anti-SNAP25 antibody (ab5666)
    Flow Cytometry - Anti-SNAP25 antibody (ab5666)

    Flow cytometry analysis of Neuro-2a cells using ab5666.Cells were fixed with 70% ethanol for 10 minutes,permeabilized with 0.25% Triton™ X-100 for 20 minutes, and blocked with 5% BSA for 30 minutes at room temperature.Cells were labeled with SNAP25 (red histogram) or with rabbit isotype control (pink histogram) at 3-5 ug/million cells in 2.5% BSA.After incubation at room temperature for 2 hours,the cells were labeled with Alexa Fluor® 488 Goat Anti-Rabbit Secondary Antibody at a dilution of 1:400 for 30 minutes at room temperature.The purple histogram represents unstained control cells and the green histogram represents no-primary-antibody control.

  • Immunocytochemistry - Anti-SNAP25 antibody (ab5666)
    Immunocytochemistry - Anti-SNAP25 antibody (ab5666)
    Immunlocalization of SNAP-25 in cultured rat hippocampal cells using ab5666.
  • Western blot - Anti-SNAP25 antibody (ab5666)
    Western blot - Anti-SNAP25 antibody (ab5666)This image is courtesy of an anonymous Abreview
    Anti-SNAP25 antibody (ab5666) at 1/10000 dilution + Whole tissue lysate prepared from mouse hippocampus.

    Secondary
    HRP conjugated goat anti-rabbit Ig. at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 25 kDa
    why is the actual band size different from the predicted?


    Exposure time: 20 seconds

    See Abreview

プロトコール

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (37)

    ab5666 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab5666 は 37 報の論文で使用されています。

    • Lauretti E  et al. Extra virgin olive oil improves synaptic activity, short-term plasticity, memory, and neuropathology in a tauopathy model. Aging Cell 19:e13076 (2020). PubMed: 31762202
    • Liu Y  et al. Eyes shut homolog (EYS) interacts with matriglycan of O-mannosyl glycans whose deficiency results in EYS mislocalization and degeneration of photoreceptors. Sci Rep 10:7795 (2020). PubMed: 32385361
    • Zhu C  et al. SARM1 deficiency up-regulates XAF1, promotes neuronal apoptosis, and accelerates prion disease. J Exp Med 216:743-756 (2019). PubMed: 30842236
    • Stévenin V  et al. Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche. Cell Rep 29:3958-3973.e7 (2019). PubMed: 31851926
    • Sifuentes-Dominguez LF  et al. SCGN deficiency results in colitis susceptibility. Elife 8:N/A (2019). PubMed: 31663849
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-6 of 6 Abreviews or Q&A

    Western blot abreview for Anti-SNAP25 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Non-reduced Denaturing (Extra PAGE ONE 5-20%)
    Sample
    Chicken Tissue lysate - whole (Brain and DRG)
    Specification
    Brain and DRG
    Blocking step
    Blocking one as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 09 2015

    Western blot abreview for Anti-SNAP25 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    5 µg
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Sample
    Rat Tissue lysate - whole (hippocampus,tissue lysate)
    Specification
    hippocampus,tissue lysate
    Treatment
    RIPA
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
    Read More

    Abcam user community

    Verified customer

    投稿 Jun 06 2014

    Question

    Please provide concentration of ab5666 lot number GR47976-2

    Read More

    Abcam community

    Verified customer

    Asked on Feb 24 2012

    Answer

    Thank you for your recent telephone enquiry.

    I can confirm that ab5666 SNAP25 antibodyis provided at 1 mg/ml. I have updated the online datasheet.

    I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

    Read More

    Abcam Scientific Support

    Answered on Feb 24 2012

    Western blot abreview for Anti-SNAP25 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (hippocampus)
    Specification
    hippocampus
    Gel Running Conditions
    Reduced Denaturing (12% TGS)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 20 2008

    Western blot abreview for Anti-SNAP25 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain)
    Loading amount
    100 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%
    Read More

    Abcam user community

    Verified customer

    投稿 Nov 17 2006

    Question

    BATCH NUMBER 137071 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM no signal by Western blot of mouse trigeminal ganglia protein extracts or immunohistochemistry on mouse brain, using recommended primary antibody dilutions SAMPLE Western blot: mouse trigeminal ganglia total protein extract PRIMARY ANTIBODY SNAP 25 (1:2000) in 1% albumin in 1xPBS containing 0.005% Tween-20, incubation: 2 hours, wash with 1xPBS containing 0.05% Tween-20 DETECTION METHOD Pierce SuperSignal West Dura Extended duration substrate POSITIVE AND NEGATIVE CONTROLS USED I am writing to request information about an appropriate positive control. ANTIBODY STORAGE CONDITIONS 20 ul aliquots stored at -20 C SAMPLE PREPARATION lysis buffer: 1% triton x-100, 2 mM EDTA in 1xPBS Calbiochem protease inhibitor cocktail 3 min boil AMOUNT OF PROTEIN LOADED 100 ug ELECTROPHORESIS/GEL CONDITIONS sample buffer: reducing conditions, specifically, contains Tris-HCl, SDS, glycerol, and B-mercaptoethanol gel: 18% Tris-HCl TRANSFER AND BLOCKING CONDITIONS blocking: 1% albumin in 1xPBS containing 0.005% Tween-20 SECONDARY ANTIBODY Pierce Goat anti-Rabbit HRP-conjugated (1:1500) x 2 hour wash with 1xPBS containing 0.005% Tween-20 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? longer antibody incubation time

    Read More

    Abcam community

    Verified customer

    Asked on Oct 31 2005

    Answer

    I'm very sorry to hear you are experiencing problems with ab5666 in both WB and IHC, this antibody has not been tested on sections (only in cell culture) and without a detailed IHC protocol it is hard to determine what the problem may be in that application. We have tested the antibody in WB on rat and mouse brain lysate and therefore with the following recommendations I hope this will improve you signal: - use a stronger lysis buffer to extract the protein (e.g RIPA buffer which contains a strong detergent) -incubate the membrane in blocking buffer (5%BSA) in TBST (Tris buffer with Tween 0.1%) for 1 hr then -incubate the membrane in antibody at 1:1000 overnight (diluted in TBST Tween 0.1%) at 4C Please rinse in TBST too and check that your secondary antibody works well with other primary antibodies. These steps will promote the antibody binding and enhance the signal. If you would like me to look at your IHC protocol I enclose a link to the IHC protocol questionnaire, if you put it to my attention I will look at this as soon as I receive it: https://www.abcam.com/index.html?section=western&pageconfig=technical&intAbID=5666&mode=questionaire Please let me know if you need further assistance,

    Read More

    Abcam Scientific Support

    Answered on Nov 01 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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