Key features and details
- Rabbit polyclonal to SNAP23
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
SNAP23 一次抗体 製品一覧
製品の詳細Rabbit polyclonal to SNAP23
特異性This antibody reacts specifically with human SNAP23.
アプリケーション適用あり: IHC-Pmore details
Fusion protein corresponding to Human SNAP23. Immunogen is a SNAP23-GST fusion protein
Database link: O00161
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab4114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
機能Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
組織特異性Ubiquitous. Highest levels where found in placenta.
配列類似性Belongs to the SNAP-25 family.
Contains 2 t-SNARE coiled-coil homology domains.
細胞内局在Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
- Information by UniProt
- HsT17016 antibody
- LS-B8340 antibody
- SNAP 23 antibody
IHC image of ab4114 staining in human placenta tissue formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4114, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ab4114 は 17 報の論文で使用されています。
- Xiong X et al. Acid Sphingomyelinase regulates the localization and trafficking of palmitoylated proteins. Biol Open 8:N/A (2019). PubMed: 31142470
- Stévenin V et al. Dynamic Growth and Shrinkage of the Salmonella-Containing Vacuole Determines the Intracellular Pathogen Niche. Cell Rep 29:3958-3973.e7 (2019). PubMed: 31851926
- Bertuccio CA et al. Plasma membrane insertion of KCa2.3 (SK3) is dependent upon the SNARE proteins, syntaxin-4 and SNAP23. PLoS One 13:e0196717 (2018). PubMed: 29768434
- Lesteberg K et al. Recycling endosomes in human cytotoxic T lymphocytes constitute an auxiliary intracellular trafficking pathway for newly synthesized perforin. Immunol Res 65:1031-1045 (2017). Flow Cyt ; Human . PubMed: 28822075
- Klein O et al. Rab5 is critical for SNAP23 regulated granule-granule fusion during compound exocytosis. Sci Rep 7:15315 (2017). PubMed: 29127297