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  1. Link

    snap23-antibody-ab3340.pdf

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Neuroscience Neurotransmission Secretory Vesicles SNAPs & SNAREs
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Anti-SNAP23 抗体 (ab3340)

  • Datasheet
  • SDS
Reviews (7) Submit a question References (16)

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Western blot - Anti-SNAP23 antibody (ab3340)
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab3340)
  • Western blot - Anti-SNAP23 antibody (ab3340)
  • Western blot - Anti-SNAP23 antibody (ab3340)
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)
  • Western blot - Anti-SNAP23 antibody (ab3340)

Key features and details

  • Rabbit polyclonal to SNAP23
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Mouse, Rat, Human, Recombinant fragment
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-SNAP23 antibody [EPR8538] (ab131242)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-SNAP23 antibody
    SNAP23 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to SNAP23
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: WB, ICC/IF, IP, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Human, Recombinant fragment
  • 免疫原

    Synthetic peptide corresponding to Mouse SNAP23 aa 193-210.
    Sequence:

    NKNRIDIANTRAKKLIDS


    (Peptide available as ab4956)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Neuroscience
    • Neurotransmission
    • Secretory Vesicles
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SNAP23 protein (ab79180)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab3340の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (6)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. 23kDa band represents SNAP 23 from rat brain protein extract.
ICC/IF
Use a concentration of 2 µg/ml.
IP (1)
Use at an assay dependent concentration.

Used at a concentration of 2 ug/ml for 1 hr (see Abreview).

IHC-P
Use a concentration of 1 µg/ml.
特記事項
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 23 kDa. 23kDa band represents SNAP 23 from rat brain protein extract.
ICC/IF
Use a concentration of 2 µg/ml.
IP
Use at an assay dependent concentration.

Used at a concentration of 2 ug/ml for 1 hr (see Abreview).

IHC-P
Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Essential component of the high affinity receptor for the general membrane fusion machinery and an important regulator of transport vesicle docking and fusion.
  • 組織特異性

    Ubiquitous. Highest levels where found in placenta.
  • 配列類似性

    Belongs to the SNAP-25 family.
    Contains 2 t-SNARE coiled-coil homology domains.
  • 細胞内局在

    Cell membrane. Cell membrane. Cell junction, synapse, synaptosome. Mainly localized to the plasma membrane.
  • Target information above from: UniProt accession O00161 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 8773 Human
    • Entrez Gene: 20619 Mouse
    • Entrez Gene: 64630 Rat
    • Omim: 602534 Human
    • SwissProt: O00161 Human
    • SwissProt: O09044 Mouse
    • SwissProt: O70377 Rat
    • Unigene: 511149 Human
    • Unigene: 245715 Mouse
    • Unigene: 14789 Rat
    see all
  • 別名

    • HsT17016 antibody
    • LS-B8340 antibody
    • SNAP 23 antibody
    • SNAP-23 antibody
    • SNAP23 antibody
    • SNAP23A antibody
    • SNAP23B antibody
    • SNP23_HUMAN antibody
    • Synaptosomal associated protein 23 antibody
    • Synaptosomal associated protein 23kDa antibody
    • Synaptosomal associated protein antibody
    • Synaptosomal-associated protein 23 antibody
    • Vesicle membrane fusion protein SNAP 23 antibody
    • Vesicle membrane fusion protein SNAP23 antibody
    • Vesicle-membrane fusion protein SNAP-23 antibody
    see all

画像

  • Western blot - Anti-SNAP23 antibody (ab3340)
    Western blot - Anti-SNAP23 antibody (ab3340)
    All lanes : Anti-SNAP23 antibody (ab3340) at 1 µg/ml

    Lane 1 : Untransfected U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysates
    Lane 2 : Non-specific scrambled siRNA transfected U-87 MG whole cell lysates
    Lane 3 : SNAP23 KO U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysates

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.25 µg/ml


    Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to SNAP23.

  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)

    Immunofluorescence analysis of SNAP-23 was performed using 90% confluent log phase U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) cells with ab3340. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab3340 at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab3340)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SNAP23 antibody (ab3340)
    ab3340 (1ug/ml) staining SNAP23 in human tonsil using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of cellular membrane compartments of the lymphatic nodules.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Western blot - Anti-SNAP23 antibody (ab3340)
    Western blot - Anti-SNAP23 antibody (ab3340)
    All lanes : Anti-SNAP23 antibody (ab3340) at 2 µg/ml

    Lane 1 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg/ml per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.4 µg/ml
  • Western blot - Anti-SNAP23 antibody (ab3340)
    Western blot - Anti-SNAP23 antibody (ab3340)
    Anti-SNAP23 antibody (ab3340) + Rat brain tissue
  • Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)
    Immunocytochemistry/ Immunofluorescence - Anti-SNAP23 antibody (ab3340)

    ICC/IF image of ab3340 stained PC-12 (Rat adrenal gland pheochromocytoma cell line) cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - Anti-SNAP23 antibody (ab3340)
    Western blot - Anti-SNAP23 antibody (ab3340)
    Anti-SNAP23 antibody (ab3340) at 1 µg/ml + Recombinant Human SNAP23 protein (ab79180) at 0.001 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 2 minutes

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (16)

ab3340 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab3340 は 16 報の論文で使用されています。

  • Osawa Y  et al. EXOC1 plays an integral role in spermatogonia pseudopod elongation and spermatocyte stable syncytium formation in mice. Elife 10:N/A (2021). PubMed: 33973520
  • Yang Y  et al. O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity. Nat Commun 11:181 (2020). PubMed: 31924761
  • Chen F  et al. ATG16L1 autophagy pathway regulates BAX protein levels and programmed cell death. J Biol Chem 295:15045-15053 (2020). PubMed: 32848017
  • Zurawski Z  et al. Disabling the Gß?-SNARE interaction disrupts GPCR-mediated presynaptic inhibition, leading to physiological and behavioral phenotypes. Sci Signal 12:N/A (2019). PubMed: 30783011
  • Søgaard D  et al. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int J Mol Sci 20:N/A (2019). PubMed: 30871020
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

Western blot abreview for Anti-SNAP23 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
African Green Monkey Cell lysate - whole cell (COS7)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
COS7
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 20 2015

Western blot abreview for Anti-SNAP23 antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Dog Cell lysate - whole cell (Madin-Darby canine kidney (MDCK) cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Madin-Darby canine kidney (MDCK) cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Oct 02 2015

Western blot abreview for Anti-SNAP23 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Cow Cell lysate - whole cell (Bovine aortic endothelial cell)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
Bovine aortic endothelial cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 26 2015

Western blot abreview for Anti-SNAP23 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (RBL-2H3)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
RBL-2H3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

投稿 Sep 08 2015

Western blot abreview for Anti-SNAP23 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T cells)
Gel Running Conditions
Reduced Denaturing
Loading amount
10 µg
Specification
HEK293T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

投稿 Jun 30 2015

Western blot abreview for Anti-SNAP23 antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (J774 macrophages)
Specification
J774 macrophages
Treatment
siRNA for 48h
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 May 28 2014

Immunoprecipitation abreview for Anti-SNAP23 antibody

Good
Abreviews
Abreviews
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (NIH 3T3)
Total protein in input
1e+006 cells
Specification
NIH 3T3
Immuno-precipitation step
Other
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Mar 22 2006

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