製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to SNAIL
  • 由来種
    Goat
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    C-RKPSDPNRKPNY

    , corresponding to N terminal amino acids 8-19 of Human SNAIL

  • ポジティブ・コントロール
    • This antibody gave a positive signal when tested against the SNAIL recombinant protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53519 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 3 µg/ml.
IHC-P Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
  • 組織特異性
    Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
  • 配列類似性
    Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 翻訳後修飾
    Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
    Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
    O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
    ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.
  • 細胞内局在
    Nucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ710H13.1 antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snail 1 homolog antibody
    • Snail 1 zinc finger protein antibody
    • SNAIL antibody
    • Snail family transcriptional repressor 1 antibody
    • Snail homolog 1 (Drosophila) antibody
    • SNAIL, Drosophila, homolog of, 1 antibody
    • SNAIL1 antibody
    • Zinc finger protein SNAI1 antibody
    see all

画像

  • All lanes : Anti-SNAIL antibody (ab53519) at 1/500 dilution

    Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
    Lane 2 : SLUG (SNAI2) Human Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Observed band size: 68 kDa (why is the actual band size different from the predicted?)


    Exposure time: 12 minutes


    Ab53519 recognizes the tagged recombinant SNAIL protein which has an expected molecular weight of 68 kDa
  • Immunohistochemical analysis of paraformaldehyde fixed mouse tissue sections, of an urethane induced lung adenocarcinoma, labelling SNAIL in the nuclei and cytoplasm. ab53519 was used at a 1/1000 dilution in 4% donkey serum for 16 hours at 4°C. Hear mediated antigen retrieval with Citrate pH6. Blocking with 4% serum for 2 hours at 25°C. Secondary antibody was a Biotinylated Anti-Goat IgG (H+L). 

    See Abreview

  • Western blot of Rat Kidney lysate (35µg protein in RIPA buffer) staining SNAIL with ab53519 at 0.1µg/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

参考文献

This product has been referenced in:
  • Chen Y  et al. Testicular orphan receptor 4 promotes tumor progression and implies poor survival through AKT3 regulation in seminoma. Cancer Sci 109:384-394 (2018). Read more (PubMed: 29197138) »
  • Wang X  et al. Therapeutic effects of conditioned medium from bone marrow-derived mesenchymal stem cells on epithelial-mesenchymal transition in A549 cells. Int J Mol Med 41:659-668 (2018). Read more (PubMed: 29207055) »

See all 39 Publications for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung adenocarcinoma induced by urethane)
Specification
lung adenocarcinoma induced by urethane
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
No
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
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投稿 Oct 21 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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