Anti-Smt3 抗体 (ab14405)
Key features and details
- Rabbit polyclonal to Smt3
- Suitable for: WB, ELISA
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
製品の概要
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製品名
Anti-Smt3 antibody -
製品の詳細
Rabbit polyclonal to Smt3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ELISAmore details -
種交差性
交差種: Saccharomyces cerevisiae -
免疫原
Recombinant protein (S. cerevisiae).
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ポジティブ･コントロール
- Most yeast cell lysates can be used as a positive control without induction or stimulation.
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特記事項
Centrifuge product if not completely clear after standing at room temperature.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 6.50
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
IgG fraction -
特記事項（精製）
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit serum. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab14405の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (2) |
1/1000. Detects a band of approximately 12 kDa.
Note: Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. |
| ELISA | (1) |
1/1000 - 1/5000.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 12 kDa. Note: Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation. |
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ELISA
1/1000 - 1/5000. |
ターゲット情報
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機能
Not known; suppressor of MIF2 mutations. -
配列類似性
Belongs to the ubiquitin family. SUMO subfamily.
Contains 1 ubiquitin-like domain. - Information by UniProt
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参照データベース
- Entrez Gene: 852122 Saccharomyces cerevisiae
- SwissProt: Q12306 Saccharomyces cerevisiae
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別名
- DmSUMO 1 antibody
- Small Ubiquitin-like modifier antibody
- SMT3 antibody
see all
画像
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Western blot - Anti-Smt3 antibody (ab14405)All lanes : Anti-Smt3 antibody (ab14405) at 1/1000 dilution
Lane 1 : 3 : 5 : 7 : 9 : 11 : 13, Lysate prepared from E.coli expressed Yeast SUMO protein and SUMO-GFP fusion protein after incubation with lysed insect cells
Lane 2 : 4 : 6 : 8 : 10 : 12 : 14, Lysate prepared from SUMO-GFP fusion protein after incubation with lysed insect cells in the presence of 2% SDS
Lysates/proteins at 2 µg per lane. -
ELISA - Anti-Smt3 antibody (ab14405)This image is courtesy of an anonymous Abreviewab14405 detecting Smt3 in Escherichia coli recombinant protein (BL21(DE3) by Direct ELISA. Wells were coated with 10µg each of diluted control protein (without SUMO fusion - Negative Controls) and target protein (with SUMO fusion protein) in bicarbonate buffer. Wells were blocked with 5% milk for 1 hour at room temperature. The samples were incubated with primary antibody (1/5000 in TBST +5% milk) for 45 minutes. An HRP-conjugated goat polyclonal to rabbit IgG (1/40000) was used as the secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab14405 は 9 報の論文で使用されています。
- Kobayashi T et al. Purification and Characterization of Double-Stranded Nucleic Acid-Dependent ATPase Activities of Tagged Dicer-Related Helicase 1 and its Short Isoform in Caenorhabditis elegans. Genes (Basel) 11:N/A (2020). PubMed: 32630243
- Gutierrez-Escribano P et al. Purified Smc5/6 Complex Exhibits DNA Substrate Recognition and Compaction. Mol Cell 80:1039-1054.e6 (2020). PubMed: 33301732
- Bauer SL et al. Helicase/SUMO-targeted ubiquitin ligase Uls1 interacts with the Holliday junction resolvase Yen1. PLoS One 14:e0214102 (2019). PubMed: 30897139
- Ranjha L et al. Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2. Commun Biol 2:174 (2019). PubMed: 31098407
- Ainscough JS et al. Cathepsin S is the major activator of the psoriasis-associated proinflammatory cytokine IL-36?. Proc Natl Acad Sci U S A 114:E2748-E2757 (2017). PubMed: 28289191
- Neiswinger J et al. Posttranslational Modification Assays on Functional Protein Microarrays. Cold Spring Harb Protoc 2016:pdb.prot087999 (2016). PubMed: 27698238
- Bermúdez-López M et al. Sgs1's roles in DNA end resection, HJ dissolution, and crossover suppression require a two-step SUMO regulation dependent on Smc5/6. Genes Dev 30:1339-56 (2016). PubMed: 27298337
- Lescasse R et al. End-joining inhibition at telomeres requires the translocase and polySUMO-dependent ubiquitin ligase Uls1. EMBO J 32:805-15 (2013). PubMed: 23417015
- Khan MA et al. Knockout of caspase-like gene, YCA1, abrogates apoptosis and elevates oxidized proteins in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 102:17326-31 (2005). WB ; Saccharomyces cerevisiae . PubMed: 16301538