Anti-smooth muscle Myosin heavy chain 11 抗体 [1G12] (ab683)
製品の概要
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製品名Anti-smooth muscle Myosin heavy chain 11 antibody [1G12]
smooth muscle Myosin heavy chain 11 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [1G12] to smooth muscle Myosin heavy chain 11
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由来種Mouse
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特異性This antibody reacts with human, mouse, and rat smooth muscle myosin heavy chain 11 (SM2). Does not cross-react with smooth muscle Myosin heavy chain 1.
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アプリケーション適用あり: WB, IHC-Fr, ICC/IF, IHC-Pmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide corresponding to Human smooth muscle Myosin heavy chain 11. The immunogen sequence exists in isoforms 3 and 4 but not isoforms 1 and 2 of the UniProt record P35749.
Sequence:RGPPPQETSQ
Database link: P35749-3
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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Concentration information loading...
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精製度Ascites
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ポリ/モノモノクローナル
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クローン名1G12
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アイソタイプIgG1
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研究分野
関連製品
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab683 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | 1/1000. PubMed: 21103378 | |
IHC-Fr | 1/400. | |
ICC/IF | 1/250. | |
IHC-P | 1/400. For paraffin embedded sections, Carnoy's fixative may give better results than formalin. |
ターゲット情報
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機能Muscle contraction.
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組織特異性Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
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関連疾患Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content. -
配列類似性Contains 1 IQ domain.
Contains 1 myosin head-like domain. -
ドメインThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2). -
細胞内局在Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
- Information by UniProt
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参照データベース
- Entrez Gene: 4629 Human
- Entrez Gene: 17880 Mouse
- Entrez Gene: 24582 Rat
- Omim: 160745 Human
- SwissProt: P35749 Human
- SwissProt: O08638 Mouse
- SwissProt: Q63862 Rat
- Unigene: 460109 Human
see all -
別名
- AAT4 antibody
- DKFZp686D10126 antibody
- DKFZp686D19237 antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [1G12] (ab683)This image is courtesy of an Abreview submitted by Dr Vyacheslav Ogay
ab683 at 1/400 staining rabbit oesophagus tissue sections by IHC-Fr. The tissue sections were paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 1 hour. An Alexa Fluor® 594 conjugated goat anti-mouse IgG1 was used as the secondary.
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Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [1G12] (ab683)This image is courtesy of an anonymous Abreview
ab683 staining smooth muscle Myosin heavy chain 11 in rat cells from cells from mesenteric artery by ICC/IF. Cells were PFA fixed and permeabilized in 0.3% Triton X-100 prior to blocking in 2% serum for 30 minutes at 20°C. The primary antibody was diluted 1/300 and incubated with the sample for 14 hours at 4°C. Alexa Fluor® 488 chicken polyclonal to mouse Ig, diluted 1/400, was used as the secondary. Nuclei stained with Hoechst 33342.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-smooth muscle Myosin heavy chain 11 antibody [1G12] (ab683)This image is courtesy of an anonymous Abreview
ab683 staining smooth muscle Myosin heavy chain 11 in human cerebral artery tissue sections by Immunohistochemistry (IHC-P - Formalin/PFA-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized with 0.3% Triton-X and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in sodium citrate. Samples were incubated with the primary antibody (1/500) for 16 hours at 4°C. A biotin-conjugated rabbit anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
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Immunocytochemistry/ Immunofluorescence - Anti-smooth muscle Myosin heavy chain 11 antibody [1G12] (ab683)This image is courtesy of an anonymous Abreview
ab683 staining smooth muscle Myosin heavy chain in human freshly isolated arterial myocyte by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 2% BSA for 30 minutes at 20°C. Samples were incubated with the primary antibody (1/300 in PBS) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated chicken anti-mouse IgG polyclonal (1/400) was used as the secondary antibody.
プロトコール
データシートおよび資料
参考文献
This product has been referenced in:
- Li Z et al. Upregulation of Oxytocin Receptor in the Hyperplastic Prostate. Front Endocrinol (Lausanne) 9:403 (2018). Read more (PubMed: 30123183) »
- Zhou B et al. Angiogenic Factor With G Patch and FHA Domains 1 Is a Novel Regulator of Vascular Injury. Arterioscler Thromb Vasc Biol 37:675-684 (2017). Read more (PubMed: 28153879) »