Anti-SMC4 抗体 (ab17958)
Key features and details
- Rabbit polyclonal to SMC4
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SMC4 antibody
SMC4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SMC4 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat, Rabbit, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant
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免疫原
Synthetic peptide representing aportion of human SMC4 lying within exon 23 (C terminal).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17958の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 150 kDa).
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| 特記事項 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 200 kDa (predicted molecular weight: 150 kDa). |
ターゲット情報
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機能
Central component of the condensin complex, a complex required for conversion of interphase chromatin into mitotic-like condense chromosomes. The condensin complex probably introduces positive supercoils into relaxed DNA in the presence of type I topoisomerases and converts nicked DNA into positive knotted forms in the presence of type II topoisomerases. -
組織特異性
Widely expressed. Higher expression in testis, colon, thymus. -
配列類似性
Belongs to the SMC family. SMC4 subfamily. -
ドメイン
The hinge domain, which separates the large intramolecular coiled coil regions, allows the heterodimerization with SMC2, forming a V-shaped heterodimer. -
細胞内局在
Nucleus. Cytoplasm. Chromosome. In interphase cells, the majority of the condensin complex is found in the cytoplasm, while a minority of the complex is associated with chromatin. A subpopulation of the complex however remains associated with chromosome foci in interphase cells. During mitosis, most of the condensin complex is associated with the chromatin. At the onset of prophase, the regulatory subunits of the complex are phosphorylated by CDC2, leading to condensin's association with chromosome arms and to chromosome condensation. Dissociation from chromosomes is observed in late telophase. - Information by UniProt
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参照データベース
- Entrez Gene: 353350 Cow
- Entrez Gene: 10051 Human
- Entrez Gene: 295107 Rat
- Entrez Gene: 702615 Rhesus monkey
- Omim: 605575 Human
- SwissProt: Q9NTJ3 Human
- Unigene: 58992 Human
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別名
- CAP C antibody
- CAPC antibody
- Chromosome associated polypeptide C antibody
see all
画像
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Western blot - Anti-SMC4 antibody (ab17958)ab17958 at 0.2
µ g/ml detecting SMC4 from 90µ g of whole cell lysate from 293T cells (left hand lane) or from normal human lymphoblasts (right hand lane) by western blot. The blot was detected by ECL. ab17958 at 0.2µg/ml detecting SMC4 from 90µg of whole cell lysate from 293T cells (left hand lane) or from normal human lymphoblasts (right hand lane) by western blot. The blot was detected by ECL.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab17958 は 10 報の論文で使用されています。
- Weyburne E & Bosco G Cancer-associated mutations in the condensin II subunit CAPH2 cause genomic instability through telomere dysfunction and anaphase chromosome bridges. J Cell Physiol 236:3579-3598 (2021). PubMed: 33078399
- Abramo K et al. A chromosome folding intermediate at the condensin-to-cohesin transition during telophase. Nat Cell Biol 21:1393-1402 (2019). PubMed: 31685986
- Xing X et al. Nesprin-2 Interacts with Condensin Component SMC2. Int J Cell Biol 2017:8607532 (2017). PubMed: 29445399
- Gerasimova T et al. A structural hierarchy mediated by multiple nuclear factors establishes IgH locus conformation. Genes Dev 29:1683-95 (2015). ChIP ; Mouse . PubMed: 26302788
- Yokoyama Y et al. A novel role for the condensin II complex in cellular senescence. Cell Cycle 14:2160-70 (2015). PubMed: 26017022
- Murakami-Tonami Y et al. Inactivation of SMC2 shows a synergistic lethal response in MYCN-amplified neuroblastoma cells. Cell Cycle 13:1115-31 (2014). PubMed: 24553121
- Pan X et al. YY1 controls Ig? repertoire and B-cell development, and localizes with condensin on the Ig? locus. EMBO J 32:1168-82 (2013). PubMed: 23531880
- Huang K et al. Ribosomal RNA gene transcription mediated by the master genome regulator protein CCCTC-binding factor (CTCF) is negatively regulated by the condensin complex. J Biol Chem 288:26067-77 (2013). WB . PubMed: 23884423
- Dávalos V et al. Human SMC2 Protein, a Core Subunit of Human Condensin Complex, Is a Novel Transcriptional Target of the WNT Signaling Pathway and a New Therapeutic Target. J Biol Chem 287:43472-81 (2012). WB ; Human . PubMed: 23095742
- Liu W et al. PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression. Nature 466:508-12 (2010). WB, ChIP-sequencing ; Human . PubMed: 20622854