Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
Yes - 0.5% Triton X100 in PBS
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Asynchronous HeLa cells were methanol fixed (-20'C, 10min), permeablized and immunofluorescently labeled. Cells were counterstained with DAPI to identify nuclei.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Oct 26 2007