Anti-SMC1A 抗体 (ab9262)
Key features and details
- Rabbit polyclonal to SMC1A
- Suitable for: IHC-P, IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SMC1A antibody
SMC1A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SMC1A -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IP, WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Cow
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免疫原
Synthetic peptide within Human SMC1A aa 1133-1233 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q14683 -
ポジティブ･コントロール
- WB: HeLa, 293T and NIH3T3 whole cell lysate. IHC-P: Human ovarian cancer tissue, mouse colon cancer tissue CT26. IP: HeLa whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Antibodies were affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at 280nm of 1.4 equals 1.0 mg of IgG. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab9262の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at 1-4 µg/mg of lysate.
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| WB | (5) |
1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa).
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| 特記事項 |
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IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at 1-4 µg/mg of lysate. |
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WB
1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 143 kDa). |
ターゲット情報
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機能
Involved in chromosome cohesion during cell cycle and in DNA repair. Central component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Involved in DNA repair via its interaction with BRCA1 and its related phosphorylation by ATM, or via its phosphorylation by ATR. Works as a downstream effector both in the ATM/NBS1 branch and in the ATR/MSH2 branch of S-phase checkpoint. -
関連疾患
Defects in SMC1A are the cause of Cornelia de Lange syndrome type 2 (CDLS2) [MIM:300590]; also known as Cornelia de Lange syndrome X-linked. CDLS is a clinically heterogeneous developmental disorder associated with malformations affecting multiple systems. CDLS is characterized by facial dysmorphisms, abnormal hands and feet, growth delay, cognitive retardation and various other malformations including gastroesophageal dysfunction and cardiac, ophthalmologic and genitourinary anomalies. -
配列類似性
Belongs to the SMC family. SMC1 subfamily. -
ドメイン
The flexible hinge domain, which separates the large intramolecular coiled coil regions, allows the heterotypic interaction with the corresponding domain of SMC3, forming a V-shaped heterodimer. The two heads of the heterodimer are then connected by different ends of the cleavable RAD21 protein, forming a ring structure. -
翻訳後修飾
Phosphorylated by ATM upon ionizing radiation in a NBS1-dependent manner. Phosphorylated by ATR upon DNA methylation in a MSH2/MSH6-dependent manner. Phosphorylation of Ser-957 and Ser-966 activates it and is required for S-phase checkpoint activation. -
細胞内局在
Nucleus. Chromosome. Chromosome > centromere > kinetochore. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. In germ cells, cohesin complex dissociates from chromatin at prophase I, and may be replaced by a meiosis-specific cohesin complex. The phosphorylated form on Ser-957 and Ser-966 associates with chromatin during G1/S/G2 phases but not during M phase, suggesting that phosphorylation does not regulate cohesin function. Integral component of the functional centromere-kinetochore complex at the kinetochore region during mitosis. - Information by UniProt
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参照データベース
- Entrez Gene: 282370 Cow
- Entrez Gene: 8243 Human
- Entrez Gene: 24061 Mouse
- Entrez Gene: 63996 Rat
- Omim: 300040 Human
- SwissProt: O97593 Cow
- SwissProt: Q14683 Human
- SwissProt: Q9CU62 Mouse
see all -
別名
- Chromosome segregation protein SmcB antibody
- DXS423E antibody
- KIAA0178 antibody
see all
画像
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Western blot - Anti-SMC1A antibody (ab9262)All lanes : Anti-SMC1A antibody (ab9262) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 143 kDa
Exposure time: 3 secondsDetection: Chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian cancer tissue, labeling SMC1 with ab9262 at 0.2 µg/mL. Detection: DAB.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SMC1A antibody (ab9262)Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse colon cancer tissue CT26, labeling SMC1 with ab9262 at 0.2 µg/mL. Detection: DAB.
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Immunoprecipitation - Anti-SMC1A antibody (ab9262)SMC1A was immunoprecipitated from 1 mg HeLa whole cell lysate with ab9262 at 6 µg per reaction. Western blot was performed on the immunoprecipitate using ab243875 at 1/1000 dilution.
Lysates prepared using NETN lysis buffer.
Lane 1: ab9262 IP in HeLa whole cell lysate.
Lane 2: Contol IgG in HeLa whole cell lysate.
Detection: Chemiluminescence.
Exposure time: 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (37)
ab9262 は 37 報の論文で使用されています。
- Li X et al. Cohesin promotes HSV-1 lytic transcription by facilitating the binding of RNA Pol II on viral genes. Virol J 18:26 (2021). PubMed: 33485391
- Bailey ML et al. Paralogous synthetic lethality underlies genetic dependencies of the cancer-mutated gene STAG2. Life Sci Alliance 4:N/A (2021). PubMed: 34462321
- Chai P et al. Generation of onco-enhancer enhances chromosomal remodeling and accelerates tumorigenesis. Nucleic Acids Res 48:12135-12150 (2020). PubMed: 33196849
- Jia L et al. Oplr16 serves as a novel chromatin factor to control stem cell fate by modulating pluripotency-specific chromosomal looping and TET2-mediated DNA demethylation. Nucleic Acids Res 48:3935-3948 (2020). PubMed: 32055844
- Reichmann J et al. Tex19.1 inhibits the N-end rule pathway and maintains acetylated SMC3 cohesin and sister chromatid cohesion in oocytes. J Cell Biol 219:N/A (2020). PubMed: 32232464