Application
Immunocytochemistry
Sample
Human Cell (HeLa and SK-N-SH)
Specification
HeLa and SK-N-SH
Fixative
Paraformaldehyde
Other product details
Dilution
1/200
Incubation time
30 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-rabbit (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
I tested this antibody in paraformaldehyde-fixed HeLa and SK-N-SH cells. In HeLa cells it gave an abnormal localization pattern and appeared to be associated with the cytoskeleton, perhaps microtubules, whereas in SK-N-SH cells it appeared to give a normal localization pattern and was associated with centrosome.
This antibody appears to work best in SK-N-SH and not HeLa cells.
This antibody appears to work best in SK-N-SH and not HeLa cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Oct 12 2005