Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoblats)
Loading amount
40 µg
Specification
lymphoblats
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris Gel, MOPS Buffer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/2500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Milk/PBST
Secondary antibody
Name
Abcam antibody:Goat anti-Rabbit IgG Fc (HRP) secondary antibody
Dilution
1/5000
Detection
Detection method
ECL
Exposure
4 minute(s) and 30 second(s)
Bands
Specific: ~60 kDa Non-specific: 49, 52, 55 kDa
Additional data
Abcam response
To reduce background ab40759 could be more diluted ab40759 e.g. 1/5000 and incubation time shortened. Secondary antibody should be diluted further too.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Lukas Rambousek
Verified customer
投稿 Dec 15 2010