Anti-Smad2 + Smad3 (phospho T8) 抗体 (ab272332)
Key features and details
- Rabbit polyclonal to Smad2 + Smad3 (phospho T8)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Smad2 + Smad3 (phospho T8) antibody
Smad2 + Smad3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Smad2 + Smad3 (phospho T8) -
由来種
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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免疫原
Synthetic peptide corresponding to Human Smad2 + Smad3 (phospho T8). Synthetic phosphopeptide derived from human Smad2/3 around the phosphorylation site of Threonine 8. Q15796,P84022.
Database link: P84022 -
ポジティブ･コントロール
- WB: HEK293T whole cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab272332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| WB |
Human
|
| All applications |
Rat
|
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000 - 1/2000.
|
| 特記事項 |
|---|
|
WB
1/1000 - 1/2000. |
ターゲット情報
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関連性
SMAD is a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. They mediate the signal of the transforming growth factor (TGF)-beta, and thus regulate multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. -
細胞内局在
Cytoplasm. Nucleus. Note: Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4. -
参照データベース
- Entrez Gene: 4087 Human
- Entrez Gene: 4088 Human
- Entrez Gene: 17126 Mouse
- Entrez Gene: 17127 Mouse
- Entrez Gene: 25631 Rat
- Entrez Gene: 29357 Rat
- Omim: 601366 Human
- Omim: 603109 Human
see all -
別名
- hMAD 2 antibody
- hMAD 3 antibody
- hSMAD2 antibody
see all
画像
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Western blot - Anti-Smad2 + Smad3 (phospho T8) antibody (ab272332)All lanes : Anti-Smad2 + Smad3 (phospho T8) antibody (ab272332) at 1/500 dilution
Lane 1 : HEK293T whole cell lysate
Lane 2 : HEK293T treated with TNFa(20ng/ml)for 10 min whole cell lysate
Lane 3 : Mouse Heart tissue lysate
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab272332 は論文での使用が確認できていません。