Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (4-12% NuPage)
Sample
Human Cell lysate - whole cell (MDA-MB-231 cells)
Specification
MDA-MB-231 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in PBST
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
Super Signal West Dura
Bands
Specific: 35 kDa Non-specific: 50, 65 kDa
Exposure
30 second(s)
Negative control
MDA-MB-231 shSlug cells.
Positive control
MDA-MB-231 cells.
Additional data
Additional Notes
Lane 1: MDA-MB-231, Lane 2: MDA-MB-231 sh Slug 1, Lane 3: MDA-MB-231 sh Slug 2, Lane 4: MDA-MB-231 sh Snail 1. The signal is completely gone using shRNA to knock down slug expression, but does not change when we knock down snail expression.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 21 2015