Anti-SLP76 抗体 (ab17029)
Key features and details
- Goat polyclonal to SLP76
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SLP76 antibody
SLP76 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SLP76 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
ALRNVPFRSEV
, corresponding to amino acids 2-12 of Human SLP76. -
ポジティブ･コントロール
- Recombinant Human SLP76 protein (ab114697) can be used as a positive control in WB.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab17029の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (1) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 61 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Involved in T-cell antigen receptor mediated signaling. -
組織特異性
Highly expressed in spleen, thymus and peripheral blood leukocytes. Highly expressed also in T-cell and monocytic cell lines, expressed at lower level in B-cell lines. Not detected in fibroblast or neuroblastoma cell lines. -
配列類似性
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 SH2 domain. -
ドメイン
The SH2 domain mediates interaction with SHB. -
翻訳後修飾
Phosphorylated after T-cell receptor activation by ZAP-70. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 3937 Human
- Omim: 601603 Human
- SwissProt: Q13094 Human
- Unigene: 304475 Human
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別名
- 76 kDa tyrosine phosphoprotein antibody
- CG8697 antibody
- LCP 2 antibody
see all
画像
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Western blot - Anti-SLP76 antibody (ab17029)Anti-SLP76 antibody (ab17029) at 0.3 µg/ml + Jurkat lysate (protein in RIPA buffer) at 35 µg
Predicted band size: 61 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Primary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (1)
ab17029 は 1 報の論文で使用されています。
- Lev A et al. Inherited SLP76 deficiency in humans causes severe combined immunodeficiency, neutrophil and platelet defects. J Exp Med 218:N/A (2021). PubMed: 33231617
