Application
Western blot
Sample
Human Tissue lysate - other (Mitochondria isolated from cortex, human frozen)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gradient gel. Laemmli buffer with 10% β-Mercaptoethanol. Samples were denatured at 55C for 10 min. Immobilon-FL transfer membranes. Overnight transfer at +4C.)
Loading amount
15 µg
Specification
Mitochondria isolated from cortex, human frozen
Blocking step
SEA Block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 22°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: SEA Block Blocking Buffer
Dilution
1/1000
Secondary antibody
Dilution
1/15000
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Odyssey CLx Imaging
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: 70 kDa
Additional data
Additional Notes
Lines on the image:
1. BioRad Precision Plus Protein standards, bands from top to bottom: 250, 150, 100, 75, 50, 37, 25, 20, 15 and 10 kDa
2 and 3. Mitochondria from cortex, human frozen tissue
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 15 2019