Anti-SLC25A12 抗体 [EPR16294] (ab200201)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR16294] to SLC25A12
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SLC25A12 antibody [EPR16294]
SLC25A12 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR16294] to SLC25A12 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human fetal heart and skeletal muscle lysates; HeLa, A431 C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse and rat brain, heart and kidney lysates. IP: Jurkat whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR16294 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab200201の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
1/40.
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WB | (1) |
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa).
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特記事項 |
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IP
1/40. |
WB
1/1000. Detects a band of approximately 75 kDa (predicted molecular weight: 75 kDa). |
ターゲット情報
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機能
Calcium-dependent mitochondrial aspartate and glutamate carrier. May have a function in the urea cycle. -
組織特異性
High levels in heart and skeletal muscle, low in brain and very low in kidney. -
関連疾患
Defects in SLC25A12 are the cause of aspartate-glutamate carrier 1 deficiency (AGC1D) [MIM:612949]; also called global cerebral hypomyelination. This syndrome consists of a child severe psychomotor retardation, hypotonia and hypomyelination of the central nervous system. -
配列類似性
Belongs to the mitochondrial carrier family.
Contains 4 EF-hand domains.
Contains 3 Solcar repeats. -
細胞内局在
Mitochondrion inner membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 8604 Human
- Entrez Gene: 78830 Mouse
- Entrez Gene: 362145 Rat
- Omim: 603667 Human
- SwissProt: O75746 Human
- SwissProt: Q8BH59 Mouse
- Unigene: 470608 Human
- Unigene: 30928 Mouse
see all -
別名
- AGC1 antibody
- Araceli hiperlarga antibody
- ARALAR antibody
see all
画像
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Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SLC25A12 knockout HAP1 cell lysate (20 µg)
Lane 3: Human skeletal muscle lysate (20 µg)
Lane 4: A431 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab200201 observed at 72 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab200201 was shown to recognize SLC25A12 when SLC25A12 knockout samples were used, along with additional cross-reactive bands. Wild-type and SLC25A12 knockout samples were subjected to SDS-PAGE. ab200201 and ab8245 (loading control to GAPDH) were diluted to 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 andGoat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
All lanes : Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/5000 dilution
Lane 1 : Human fetal heart lysate
Lane 2 : Human skeletal muscle lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lane 2 : A431 (Human epidermoid carcinoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-SLC25A12 antibody [EPR16294] (ab200201) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lane 6 : Rat kidney lysate
Lane 7 : C6 (Rat glial tumor cells) whole cell lysate
Lane 8 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 9 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
Lane 10 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 75 kDa
Observed band size: 75 kDa
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution.
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab200201 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 second. -
SLC25A12 was immunoprecipitated from 1mg of Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate with ab200201 at 1/40 dilution.
Western blot was performed from the immunoprecipitate using ab200201 at 1/1000 dilution (Panel A) or ab192244 at 1/1000 dilution (Panel B).
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: Jurkat whole cell lysate 10 µg (Input).
Lane 2: ab200201 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab200201 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time (Panel A and Panel B): 30 second.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab200201 は 2 報の論文で使用されています。
- Yasukawa K et al. The microRNAs miR-302b and miR-372 regulate mitochondrial metabolism via the SLC25A12 transporter, which controls MAVS-mediated antiviral innate immunity. J Biol Chem 295:444-457 (2020). PubMed: 31767682
- Sen NE et al. Generation of an Atxn2-CAG100 knock-in mouse reveals N-acetylaspartate production deficit due to early Nat8l dysregulation. Neurobiol Dis 132:104559 (2019). PubMed: 31376479