Anti-SLC14A2 抗体 (ab95365)
Key features and details
- Rabbit polyclonal to SLC14A2
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-SLC14A2 antibody
SLC14A2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SLC14A2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat
交差が予測される動物種: Human -
免疫原
Synthetic peptide corresponding to the C terminal amino acids 911-929 (
QEKNRRASMITKYQAYDVS
) of Rat SLC14A2 (NP_062220). -
ポジティブ・コントロール
- IHC-P: Rat colon tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95365の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 102 kDa. 1 µg/ml of ab95365 was sufficient for detection of SLC14A2 in 20µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.
|
|
IHC-P |
1/25.
|
特記事項 |
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WB
1/1000. Predicted molecular weight: 102 kDa. 1 µg/ml of ab95365 was sufficient for detection of SLC14A2 in 20µg of rat kidney tissue lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody. |
IHC-P
1/25. |
ターゲット情報
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機能
Specialized low-affinity vasopressin-regulated urea transporter. Mediates rapid transepithelial urea transport across the inner medullary collecting duct and plays a major role in the urinary concentrating mechanism. -
組織特異性
Isoform 1 and isoform 2 are expressed in the inner medulla of the kidney. -
配列類似性
Belongs to the urea transporter family. -
細胞内局在
Apical cell membrane. - Information by UniProt
-
参照データベース
- Entrez Gene: 8170 Human
- Entrez Gene: 54302 Rat
- Omim: 601611 Human
- SwissProt: Q15849 Human
- SwissProt: Q62668 Rat
- Unigene: 710927 Human
- Unigene: 10157 Rat
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別名
- FLJ16167 antibody
- hUT-A6 antibody
- HUT2 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded formalin fixed (4%) rat kidney tissue labelling SLC14A2 with ab95365 at 1/25 dilution for 1 hour at RT, followed by secondary antibody Goat Anti-Rabbit IgG (Alexa Fluor 488) (Green). Membrane staining on rat kidney tissue is observed. Counter stained with Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 (Red) F-Actin stain. (C) UT-A1 Antibody (D) Composite image.
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Western blot detection of SLC14A2 in Rat inner medulla using ab95365 at 1:1000 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab95365 は 1 報の論文で使用されています。
- Laszczyk AM et al. Pax2 and Pax8 Proteins Regulate Urea Transporters and Aquaporins to Control Urine Concentration in the Adult Kidney. J Am Soc Nephrol 31:1212-1225 (2020). PubMed: 32381599