Anti-SLAMF6 抗体 [EPR23122-130] (ab243658)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23122-130] to SLAMF6
- Suitable for: ICC/IF, Flow Cyt, IP
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-SLAMF6 antibody [EPR23122-130]
SLAMF6 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23122-130] to SLAMF6 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, Flow Cyt, IPmore details
適用なし: IHC-P or WB -
種交差性
交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- ICC/IF: EL4 and mouse splenocytes cells. Flow Cyt: Mouse splenocytes cells. IP: Mouse spleen lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23122-130 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab243658の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/50.
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Flow Cyt |
1/500.
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IP |
1/30.
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特記事項 |
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ICC/IF
1/50. |
Flow Cyt
1/500. |
IP
1/30. |
ターゲット情報
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機能
Triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors. -
組織特異性
Expressed by all (resting and activated) natural killer cells (NK), T- and B-lymphocytes. -
配列類似性
Contains 1 Ig-like (immunoglobulin-like) domain. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 30925 Mouse
- SwissProt: Q9ET39 Mouse
- Unigene: 486404 Mouse
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別名
- Activating NK receptor antibody
- CD352 antibody
- KALI antibody
see all
画像
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SLAMF6 was immunoprecipitated from 0.35 mg Mouse spleen lysate with ab243658 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab243658 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Lane 1: Mouse spleen lysate 10µg
Lane 2: ab243658 IP in Mouse spleen lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab243658 in Mouse spleen lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 min
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Flow cytometric analysis of Mouse splenocytes cells labelling SLAMF6 with ab243658 at 1/500 compared with a Rabbit monoclonal IgG (ab172730, Left) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse splenocytes cells labelling SLAMF6 with ab243658 at 1/50 dilution, followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in some subsets of mouse splenocytes is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 dilution.
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Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized EL4 (mouse lymphoma T lymphocyte) cells labelling SLAMF6 with ab243658 at 1/50 dilution, followed by ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in EL4 cells is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is ab150077 Alexa Fluor® 488 Goat anti-Rabbit secondary at 1/1000 dilution.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab243658 は論文での使用が確認できていません。