Anti-SLAM / CD150 抗体 [IPO-3] (ab2604)

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Key features and details

  • Mouse monoclonal [IPO-3] to SLAM / CD150
  • Suitable for: ELISA, IHC-Fr, Flow Cyt, IP
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-SLAM / CD150 antibody [IPO-3]
    SLAM / CD150 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [IPO-3] to SLAM / CD150

  • 由来種

    Mouse

  • 特異性

    This antibody is specific for SLAM.

  • アプリケーション

    適用あり: ELISA, IHC-Fr, Flow Cyt, IPmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Activated B cell line

  • エピトープ

    The exact epitope recognised by this antibody has not been mapped.

  • 特記事項


    A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 一次抗体 備考

    A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.

  • ポリ/モノ

    モノクローナル

  • クローン名

    IPO-3

  • ミエローマ

    unknown

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab2604 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/750.
IHC-Fr 1/100.
Flow Cyt 1/750.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能

    High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.

  • 組織特異性

    Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.

  • 配列類似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • ドメイン

    The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.

  • 翻訳後修飾

    Phosphorylated by FYN.

  • 細胞内局在

    Cell membrane. Present on the surface of B-cells and T-cells.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 4933415F16 antibody
      • AA177906 antibody
      • CD 150 antibody
      • CD150 antibody
      • CD150 antigen antibody
      • CDw150 antibody
      • Estm51 antibody
      • Ipo 3 antibody
      • IPO-3 antibody
      • Ipo3 antibody
      • MGC151472 antibody
      • MGC151476 antibody
      • OTTHUMP00000025670 antibody
      • OTTHUMP00000060252 antibody
      • RGD1560634 antibody
      • Signaling lymphocytic activation molecule antibody
      • Signaling lymphocytic activation molecule family member 1 antibody
      • Signaling lymphocytic activation molecule family member 1, isoform CRA_a antibody
      • Signaling lymphocytic activation molecule family member 1, isoform CRA_b antibody
      • Signaling lymphocytic activation molecule precursor antibody
      • Slaf1 antibody
      • SLAF1_HUMAN antibody
      • SLAM family, member 1 antibody
      • Slamf 1 antibody
      • SLAMF1 antibody
      • SLAMF1 protein antibody
      see all

    参考文献 (5)

    ab2604 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2604 は 5 報の論文で使用されています。

    • Borel F  et al. Survival Advantage of Both Human Hepatocyte Xenografts and Genome-Edited Hepatocytes for Treatment of a-1 Antitrypsin Deficiency. Mol Ther 25:2477-2489 (2017). PubMed: 29032169
    • Currier MG  et al. EGFR Interacts with the Fusion Protein of Respiratory Syncytial Virus Strain 2-20 and Mediates Infection and Mucin Expression. PLoS Pathog 12:e1005622 (2016). PubMed: 27152417
    • Mateo M  et al. The measles virus hemagglutinin ß-propeller head ß4-ß5 hydrophobic groove governs functional interactions with nectin-4 and CD46 but not those with the signaling lymphocytic activation molecule. J Virol 87:9208-16 (2013). PubMed: 23760251
    • Avota E  et al. DC-SIGN Mediated Sphingomyelinase-Activation and Ceramide Generation Is Essential for Enhancement of Viral Uptake in Dendritic Cells. PLoS Pathog 7:e1001290 (2011). ICC/IF ; Human . PubMed: 21379338
    • Noyce RS  et al. Tumor Cell Marker PVRL4 (Nectin 4) Is an Epithelial Cell Receptor for Measles Virus. PLoS Pathog 7:e1002240 (2011). PubMed: 21901103

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Inquiry: I've often seen SLAM CD150 referred to as a 70-75 KDa protein including on this web-page. Where does this information come from? The human SLAM CD150 protein is much smaller than this at about 330 amino acids? Am I missing something?

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    Answer



    You are correct the calculated molecular weight of SLAM is ca 37 kDa.

    Modification can significantly increase the molecular weight of proteins. SLAM is a sialylated phosphoglycoprotein.

    The effect the glycosylation has on the molecular weight of SLAM is nicely demonstrated in the following publication:

    Castro., A.G., et al. (1999). J Immunol. 163(11):5860-5870 (also attached FYI)

    Therefore if the protein is modified we expect is to run at ca 50-80 kDa depending on the extent of the modification.

    Read More

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