製品の概要
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製品名Anti-SLAM / CD150 antibody [IPO-3]
SLAM / CD150 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [IPO-3] to SLAM / CD150
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由来種Mouse
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特異性This antibody is specific for SLAM.
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アプリケーション適用あり: ICC/IF, ELISA, IHC-Fr, Flow Cyt, IPmore details
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種交差性交差種: Human
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免疫原
Activated B cell line
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エピトープThe exact epitope recognised by this antibody has not been mapped.
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特記事項
A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPBS pH 7.2, 0.01% sodium azide
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Concentration information loading... -
精製度Protein A purified
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一次抗体 備考A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.
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ポリ/モノモノクローナル
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クローン名IPO-3
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ミエローマunknown
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アイソタイプIgG1
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軽鎖の種類unknown
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研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab2604 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| ICC/IF | Use at an assay dependent concentration. PubMed: 21379338 | |
| ELISA | 1/750. | |
| IHC-Fr | 1/100. | |
| Flow Cyt | 1/750. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| IP | Use at an assay dependent concentration. |
ターゲット情報
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機能High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.
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組織特異性Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.
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配列類似性Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
ドメインThe most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.
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翻訳後修飾Phosphorylated by FYN.
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細胞内局在Cell membrane. Present on the surface of B-cells and T-cells.
- Information by UniProt
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参照データベース
- Entrez Gene: 6504 Human
- Omim: 603492 Human
- SwissProt: Q13291 Human
- Unigene: 523660 Human
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別名
- 4933415F16 antibody
- AA177906 antibody
- CD 150 antibody
see all
画像
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![Immunocytochemistry/ Immunofluorescence - Anti-SLAM / CD150 antibody [IPO-3] (ab2604)](https://www.abcam.co.jp/ps/datasheet/images/2/ab2604/SLAM-CD150-Primary-antibodies-ab2604-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SLAM / CD150 antibody [IPO-3] (ab2604)Image from Avota E et al., PLoS Pathog. 2011 Feb;7(2):e1001290. Epub 2011 Feb 17. Fig 5.; doi:10.1371/journal.ppat.1001290; February 17, 2011, PLoS Pathog 7(2): e1001290.ab2604, staining SLAM / CD150 in Raji cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100 before being incubated with primary antibody. A fluorescence-conjugated anti-mouse IgG was used as the secondary antibody.
プロトコール
参考文献
This product has been referenced in:
- Currier MG et al. EGFR Interacts with the Fusion Protein of Respiratory Syncytial Virus Strain 2-20 and Mediates Infection and Mucin Expression. PLoS Pathog 12:e1005622 (2016). Read more (PubMed: 27152417) »
- Avota E et al. DC-SIGN Mediated Sphingomyelinase-Activation and Ceramide Generation Is Essential for Enhancement of Viral Uptake in Dendritic Cells. PLoS Pathog 7:e1001290 (2011). ICC/IF ; Human . Read more (PubMed: 21379338) »
レビューと Q&A
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