Anti-SLAM / CD150 抗体 [IPO-3] (ab2604)
Key features and details
- Mouse monoclonal [IPO-3] to SLAM / CD150
- Suitable for: ELISA, IHC-Fr, IP, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SLAM / CD150 antibody [IPO-3]
SLAM / CD150 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [IPO-3] to SLAM / CD150 -
由来種
Mouse -
特異性
This antibody is specific for SLAM. -
アプリケーション
適用あり: ELISA, IHC-Fr, IP, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Tissue, cells or virus corresponding to SLAM/ CD150. Activated B cell line
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エピトープ
The exact epitope recognised by this antibody has not been mapped. -
特記事項
A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide -
Concentration information loading... -
精製度
Protein A purified -
一次抗体 備考
A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells. -
ポリ/モノ
モノクローナル -
クローン名
IPO-3 -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2604の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ELISA |
Use at an assay dependent concentration.
|
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| IHC-Fr |
Use at an assay dependent concentration.
|
|
| IP |
Use at an assay dependent concentration.
|
|
| Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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| ICC/IF |
Use at an assay dependent concentration. PubMed: 21379338
|
| 特記事項 |
|---|
|
ELISA
Use at an assay dependent concentration. |
|
IHC-Fr
Use at an assay dependent concentration. |
|
IP
Use at an assay dependent concentration. |
|
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use at an assay dependent concentration. PubMed: 21379338 |
ターゲット情報
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機能
High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. -
組織特異性
Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. -
配列類似性
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
ドメイン
The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues. -
翻訳後修飾
Phosphorylated by FYN. -
細胞内局在
Cell membrane. Present on the surface of B-cells and T-cells. - Information by UniProt
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参照データベース
- Entrez Gene: 6504 Human
- Omim: 603492 Human
- SwissProt: Q13291 Human
- Unigene: 523660 Human
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別名
- 4933415F16 antibody
- AA177906 antibody
- CD 150 antibody
see all
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab2604 は 6 報の論文で使用されています。
- Tang Q et al. Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model. Methods Mol Biol 2164:75-85 (2020). PubMed: 32607885
- Borel F et al. Survival Advantage of Both Human Hepatocyte Xenografts and Genome-Edited Hepatocytes for Treatment of a-1 Antitrypsin Deficiency. Mol Ther 25:2477-2489 (2017). PubMed: 29032169
- Currier MG et al. EGFR Interacts with the Fusion Protein of Respiratory Syncytial Virus Strain 2-20 and Mediates Infection and Mucin Expression. PLoS Pathog 12:e1005622 (2016). PubMed: 27152417
- Mateo M et al. The measles virus hemagglutinin ß-propeller head ß4-ß5 hydrophobic groove governs functional interactions with nectin-4 and CD46 but not those with the signaling lymphocytic activation molecule. J Virol 87:9208-16 (2013). PubMed: 23760251
- Avota E et al. DC-SIGN Mediated Sphingomyelinase-Activation and Ceramide Generation Is Essential for Enhancement of Viral Uptake in Dendritic Cells. PLoS Pathog 7:e1001290 (2011). ICC/IF ; Human . PubMed: 21379338
- Noyce RS et al. Tumor Cell Marker PVRL4 (Nectin 4) Is an Epithelial Cell Receptor for Measles Virus. PLoS Pathog 7:e1002240 (2011). PubMed: 21901103
