Anti-SLAM / CD150 抗体 [IPO-3] (ab2604)

Submit a review Q&A (1)References (6)

Key features and details

  • Mouse monoclonal [IPO-3] to SLAM / CD150
  • Suitable for: ELISA, IHC-Fr, IP, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-SLAM / CD150 antibody [EPR5470] (ab134044)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-SLAM / CD150 antibody [IPO-3]
    SLAM / CD150 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [IPO-3] to SLAM / CD150

  • 由来種

    Mouse

  • 特異性

    This antibody is specific for SLAM.

  • アプリケーション

    適用あり: ELISA, IHC-Fr, IP, Flow Cyt, ICC/IFmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Tissue, cells or virus corresponding to SLAM/ CD150. Activated B cell line

  • エピトープ

    The exact epitope recognised by this antibody has not been mapped.

  • 特記事項


    A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
  • Concentration information loading...

  • 精製度

    Protein A purified

  • 一次抗体 備考

    A novel glycoprotein of 70kDa, designated SLAM (Signaling Lymphocyte Activation Molecule) / CDw150, that belongs to the immunoglobulin gene superfamily and is involved in T-cell stimulation. SLAM is constitutively expressed on peripheral blood memory T cells, T-cell clones, immature thymocytes, and a proportion of B cells, and is rapidly induced on naive T cells after activation. Activated B cells express the membrane-bound form of SLAM and the soluble and cytoplasmic isoforms of SLAM, and that the expression levels of membrane-bound SLAM on B cells are rapidly regulated after activation in vitro. It is suggested that signaling through homophilic SLAM-SLAM binding during B-B and B-T cell interactions enhances the expansion and differentiation of activated B cells.

  • ポリ/モノ

    モノクローナル

  • クローン名

    IPO-3

  • ミエローマ

    unknown

  • アイソタイプ

    IgG1

  • 軽鎖の種類

    unknown

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2604の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration. PubMed: 21379338
特記事項
ELISA
Use at an assay dependent concentration.
IHC-Fr
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF
Use at an assay dependent concentration. PubMed: 21379338

ターゲット情報

  • 機能

    High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates.

  • 組織特異性

    Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation.

  • 配列類似性

    Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • ドメイン

    The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues.

  • 翻訳後修飾

    Phosphorylated by FYN.

  • 細胞内局在

    Cell membrane. Present on the surface of B-cells and T-cells.
  • Information by UniProt

  • 参照データベース

    • 別名

      • 4933415F16 antibody
      • AA177906 antibody
      • CD 150 antibody
      • CD150 antibody
      • CD150 antigen antibody
      • CDw150 antibody
      • Estm51 antibody
      • Ipo 3 antibody
      • IPO-3 antibody
      • Ipo3 antibody
      • MGC151472 antibody
      • MGC151476 antibody
      • OTTHUMP00000025670 antibody
      • OTTHUMP00000060252 antibody
      • RGD1560634 antibody
      • Signaling lymphocytic activation molecule antibody
      • Signaling lymphocytic activation molecule family member 1 antibody
      • Signaling lymphocytic activation molecule family member 1, isoform CRA_a antibody
      • Signaling lymphocytic activation molecule family member 1, isoform CRA_b antibody
      • Signaling lymphocytic activation molecule precursor antibody
      • Slaf1 antibody
      • SLAF1_HUMAN antibody
      • SLAM family, member 1 antibody
      • Slamf 1 antibody
      • SLAMF1 antibody
      • SLAMF1 protein antibody
      see all

    参考文献 (6)

    ab2604 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2604 は 6 報の論文で使用されています。

    • Tang Q  et al. Engraftment of Human Hepatocytes in the PiZ-NSG Mouse Model. Methods Mol Biol 2164:75-85 (2020). PubMed: 32607885
    • Borel F  et al. Survival Advantage of Both Human Hepatocyte Xenografts and Genome-Edited Hepatocytes for Treatment of a-1 Antitrypsin Deficiency. Mol Ther 25:2477-2489 (2017). PubMed: 29032169
    • Currier MG  et al. EGFR Interacts with the Fusion Protein of Respiratory Syncytial Virus Strain 2-20 and Mediates Infection and Mucin Expression. PLoS Pathog 12:e1005622 (2016). PubMed: 27152417
    • Mateo M  et al. The measles virus hemagglutinin ß-propeller head ß4-ß5 hydrophobic groove governs functional interactions with nectin-4 and CD46 but not those with the signaling lymphocytic activation molecule. J Virol 87:9208-16 (2013). PubMed: 23760251
    • Avota E  et al. DC-SIGN Mediated Sphingomyelinase-Activation and Ceramide Generation Is Essential for Enhancement of Viral Uptake in Dendritic Cells. PLoS Pathog 7:e1001290 (2011). ICC/IF ; Human . PubMed: 21379338
    • Noyce RS  et al. Tumor Cell Marker PVRL4 (Nectin 4) Is an Epithelial Cell Receptor for Measles Virus. PLoS Pathog 7:e1002240 (2011). PubMed: 21901103

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    Inquiry: I've often seen SLAM CD150 referred to as a 70-75 KDa protein including on this web-page. Where does this information come from? The human SLAM CD150 protein is much smaller than this at about 330 amino acids? Am I missing something?

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    Answer



    You are correct the calculated molecular weight of SLAM is ca 37 kDa.

    Modification can significantly increase the molecular weight of proteins. SLAM is a sialylated phosphoglycoprotein.

    The effect the glycosylation has on the molecular weight of SLAM is nicely demonstrated in the following publication:

    Castro., A.G., et al. (1999). J Immunol. 163(11):5860-5870 (also attached FYI)

    Therefore if the protein is modified we expect is to run at ca 50-80 kDa depending on the extent of the modification.

    Read More

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