Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citric buffer, EDTA
Permeabilization
No
Specification
skin
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% donkey serum
Concentration
20 µg/ml
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Donkey anti-Goat IgG (H+L) Cross-Adsorbed
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Additional data
Additional Notes
Tried different pH buffers forantigen retrieval.
Unspecific binding-the same epitopes bound on isotype control.
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested for cross reactivity with mouse or in IHC and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested species/application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 12 2018