Anti-SKI 抗体 - C-terminal (ab226208)
Key features and details
- Rabbit polyclonal to SKI - C-terminal
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SKI antibody - C-terminal
SKI 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SKI - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide within Human SKI aa 678-728 (C terminal). The exact sequence is proprietary. NP_003027.1
Database link: P12755 -
ポジティブ・コントロール
- IP: HEK-293T whole cell lysate. WB: HEK-293T, Jurkat and HeLa whole cell lysate (ab150035).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab226208の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at 2-10 µg/mg of lysate.
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WB |
1/2000 - 1/10000. Predicted molecular weight: 80 kDa.
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特記事項 |
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IP
Use at 2-10 µg/mg of lysate. |
WB
1/2000 - 1/10000. Predicted molecular weight: 80 kDa. |
ターゲット情報
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機能
May play a role in terminal differentiation of skeletal muscle cells but not in the determination of cells to the myogenic lineage. Functions as a repressor of TGF-beta signaling. -
配列類似性
Belongs to the SKI family. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6497 Human
- Omim: 164780 Human
- SwissProt: P12755 Human
- Unigene: 656507 Human
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別名
- Avian sarcoma viral (v ski) oncogene homolog antibody
- C oncogene antibody
- C ski antibody
see all
画像
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All lanes : Anti-SKI antibody - C-terminal (ab226208) at 0.04 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 15 µg
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 80 kDa
Exposure time: 3 minutes -
SKI was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226208 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226208 at 1 µg/ml.
Lane 1: ab226208 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab226208 は 1 報の論文で使用されています。
- Zhang H et al. Overexpression of c-Ski promotes cell proliferation, invasion and migration of gastric cancer associated fibroblasts. Kaohsiung J Med Sci 35:214-221 (2019). PubMed: 30896889