SIRT6 Activity Assay Kit (Fluorometric) (ab156068)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture extracts, Tissue Extracts
製品の概要
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製品名
SIRT6 Activity Assay Kit (Fluorometric)
SIRT6 キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Cell culture extracts, Tissue Extracts -
アッセイタイプ
Enzyme activity -
全工程の試験時間
1h 00m -
種交差性
交差種: Human -
製品の概要
Abcam’s SIRT6 Activity Assay Kit (Fluorometric) (ab156068) detects deacetylase activity of recombinant SIRT6.
Primarily, the SIRT6 Activity Assay Kit (Fluorometric) is designed for the rapid and sensitive evaluation of SIRT6 inhibitors or activators using recombinant SIRT6 or purified SIRT6.
Applications for this kit include:
1. Screening inhibitors or activators of SIRT6.
2. Detecting the effects of pharmacological agents on SIRT6.
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特記事項
Histone Deacetylases (HDACs) are a class of enzymes responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), allowing the histones to wrap the DNA more tightly.
HDAC proteins occur in four groups (class I, class IIA, class IIB, class III, class IV) based on function and DNA sequence similarity.
Classes I, IIA and IIB are considered "classical" HDACs whose activities are inhibited by trichostatin A (TSA), whereas class III is a family of NAD+-dependent proteins (sirtuins) not affected by TSA. Class IV is considered an atypical class on its own, based solely on DNA sequence similarity to the others. -
試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 100 tests 100 tests Developer 1 x 500µl 1 x 500µl Fluoro-Deacetylated Peptide (0.1 mM) 1 x 100µl 1 x 100µl Fluoro-Substrate Peptide (0.1 mM) 1 x 500µl 1 x 500µl NAD (8 mM) 1 x 500µl 1 x 500µl Recombinant SIRT6 1 x 500µl 1 x 500µl SIRT Assay Buffer 2 x 1ml 2 x 1ml Stop Solution 2 x 1ml 2 x 1ml -
研究分野
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機能
NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. -
配列類似性
Belongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain. -
細胞内局在
Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions. - Information by UniProt
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別名
- 2810449N18Rik
- AI043036
- Mono ADP ribosyltransferase sirtuin 6
see all -
参照データベース
- Entrez Gene: 51548 Human
- Omim: 606211 Human
- SwissProt: Q8N6T7 Human
- Unigene: 423756 Human
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab156068 は 7 報の論文で使用されています。
- Minic Z et al. Phosphoproteomic Analysis of Breast Cancer-Derived Small Extracellular Vesicles Reveals Disease-Specific Phosphorylated Enzymes. Biomedicines 10:N/A (2022). PubMed: 35203617
- Xie T et al. Luteolin suppresses TNF-a-induced inflammatory injury and senescence of nucleus pulposus cells via the Sirt6/NF-?B pathway. Exp Ther Med 24:469 (2022). PubMed: 35747154
- Cacciola NA et al. Buffalo Milk Whey Activates Necroptosis and Apoptosis in a Xenograft Model of Colorectal Cancer. Int J Mol Sci 23:N/A (2022). PubMed: 35955595
- Li W et al. Downregulation of hippocampal SIRT6 activates AKT/CRMP2 signaling and ameliorates chronic stress-induced depression-like behavior in mice. Acta Pharmacol Sin 41:1557-1567 (2020). PubMed: 32265492
- Peng L et al. Deacetylase-independent function of SIRT6 couples GATA4 transcription factor and epigenetic activation against cardiomyocyte apoptosis. Nucleic Acids Res 48:4992-5005 (2020). PubMed: 32239217
- Yao ZQ et al. A novel small-molecule activator of Sirtuin-1 induces autophagic cell death/mitophagy as a potential therapeutic strategy in glioblastoma. Cell Death Dis 9:767 (2018). PubMed: 29991742
- Balestrieri ML et al. Sirtuin 6 expression and inflammatory activity in diabetic atherosclerotic plaques: effects of incretin treatment. Diabetes 64:1395-406 (2015). PubMed: 25325735