Anti-SIRT4 抗体 (ab10140)

That is correct. The antibody is goat immunoglobulin, purified from goat serum. The immunogen is derived from human Sirt4, but the antibody itself is only goat IgG. If you or your customer still have any questions, please contact us.

Thanks again for contacting us with your question, and for your patience. The peptide for ab49173 is now available on our website as ab123888- https://www.abcam.com/SIRT5-peptide-ab123888.html Unfortunately the peptide for ab78977 is not available for sale. I am sorry that we don't have all of the products you are looking for, but please let me know if you have any questions or if there is anything else that we can do for you.  

Thank you for contacting us with your question. We do have the blocking peptide for the SIRT5 antibody ab49173, but it isn't on our website at this point so I'm working to have this added. I'll have to check with the lab about the peptide for the SIRT7 antibody ab78977, and I'll get back to you once I have more information about its availability. Unfortunately, the peptide for the SIRT1 antibody ab32441 is not available for sale. I will be in touch once I hear about the ab49173 and ab78977 peptides, but please let me know if you have any questions in the meantime.

Thank you for kindly confirming these details as this enables us to closely monitor the quality of our products. We would be pleased to arrange a replacement product free of charge. I am forwarding these details on to our Distributor’s Team who would be happy to organize this. Distributor name:Bita Lifescience  Original order number:xxxabID of original product: 1 X vial ab78982 Original batch number:1062565  abID of replacement product: ab10140 CCEID:xxxThank you for your help and cooperation. Please do not hesitate to contact us if you need anything further.

Thank you very much for confirming these details. I would be pleased to replace the antibody with an Sirt4 antibody. If the customer is interested in an antibody for IHC-P, I agree that ab10140, ab105039 and ab115517 are suitable. As mentioned on the datasheet all of these require heat-mediated antigen retrieval. This should preferably performed with citrate buffer at pH6, however, might require optimisation according to the experimental setting, especially in the case of staining PFA-perfused mouse brain sections. As for the Sirt5 antibody ab78982, we do not know if these Sirt4 antibodies would work with a protocol for free floating sections and thus the protocol might require optimisation. Also, these antibodies have so far only been tested with human (and rat) samples, however, due to a high sequence homology we expect them to cross-react with murine Sirt4 as well. Therefore, if the customer is happy to try ab10140 (or one of the others) despite these uncertainties, I would be pleased to arrange for a replacement. Otherwise, I could issue a credit note or refund if preferred. Thank you for your cooperation. I look forward to hearing from you with details of how your customer would like to proceed.

Thank you for your customer's protocol details, I enclose below my recommendations to increase the signal, these are very similar to those given for the other antibodies the customer is having problems with: -as discussed before the fact the customer is having the correct results with over-expressed protein reassures us that the antibody works and that in Hek293T cell lysate the protein levels are much lower. I would recommend running the positive control of human kidney and U937 lysates (the customer's RIPA buffer is a good choice). -check the protease inhibitors in the lysis buffer are fresh and not old and that samples are kept on ice at all times- can I also check that the samples are left to lyse for 1hr before spinning in the centrifuge (to give time for the NP40 to permeabilise and lyse the cells)? -milk can prevent binding of the antibody to the protein and I would therefore recommend blocking the membrane in 5% BSA (30min-1hrs) and incubating the antibody in TBST only. -please try 1:300 dilution with overnight incubation. We have used 1ug/ml (i.e 1:500) in our positive control but this needs optimisation between samples. - use Ecl+ or even more sensitive kits (e.g. West Pico, West dura pierce kits) as ECL is not very sensitive -check the secondary antibody is not too old and not loosing its sensitivity, if possible by trying a different secondary antibody. I hope these suggestions will help,

Thank you for contacting us for technical support. The fact the customer is having the correct results with over-expressed protein reassures us that the antibody works and that in Hek293T cell lysate the protein levels are much lower. We have not had any complaints about these antibodies and they work very well in the tested positive controls. I would recommend running the positive controls detailed on the datasheets: ab10140: human kidney cell lysate, U937 lysate ab19388: 3T3 cell lysate ab13860: no cell line has been tested- please try human testis lysate ab13697: no cell line has been tested- please try human intestine lysate If you require further assistance can you please ask your customer to fill in our protocol questionnaires and we can look at each antibody condition; typically please check your customer incubates at the recommended dilution in TBST overnight (he/she may need to use more concentrated antibody as this depends on the tissue), please check that degradation of the protein is not occurring and other protocol details which may make a difference,