リコンビナントRabMAb

Anti-SIRT3 抗体 [EPR23514-36] - BSA and Azide free (ab270523)

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Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)
  • Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)
  • Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23514-36] to SIRT3 - BSA and Azide free
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat

製品の概要

  • 製品名

    Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free
    SIRT3 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23514-36] to SIRT3 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Rat
    See all applications and species data

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Mouse heart, liver and kidney tissue lysate; Rat liver and kidney tissue lysate. IP: Mouse and rat liver lysate.

  • 特記事項

    ab270523 is the carrier-free version of ab246522. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab270523 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
IP
Rat
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 43 kDa.
IP
Use at an assay dependent concentration.

ターゲット情報

  • 機能

    NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5O. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels.

  • 組織特異性

    Widely expressed.

  • 配列類似性

    Belongs to the sirtuin family. Class I subfamily.
    Contains 1 deacetylase sirtuin-type domain.

  • 翻訳後修飾

    Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.

  • 細胞内局在

    Mitochondrion matrix.
  • Information by UniProt

  • 参照データベース

    • 別名

      • hSIRT 3 antibody
      • hSIRT3 antibody
      • Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase antibody
      • NAD dependent deacetylase sirtuin 3 mitochondrial antibody
      • NAD-dependent protein deacetylase sirtuin-3, mitochondrial antibody
      • Regulatory protein SIR2 homolog 3 antibody
      • Silent mating type information regulation 2 S.cerevisiae homolog 3 antibody
      • Sir 2 like 3 antibody
      • SIR 2 like protein 3 antibody
      • SIR 3 antibody
      • SIR2 L3 antibody
      • Sir2 like 3 antibody
      • SIR2 like protein 3 antibody
      • SIR2-like protein 3 antibody
      • SIR2L3 antibody
      • SIR3_HUMAN antibody
      • SIRT 3 antibody
      • SIRT3 antibody
      • Sirtuin 3 antibody
      • Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae) antibody
      • Sirtuin type 3 antibody
      • Sirtuin3 antibody
      see all

    画像

    • Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)
      Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)

      SIRT3 was immunoprecipitated from 0.35 mg mouse liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse liver lysate 10μg
      Lane 2: ab246522 IP in mouse liver lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246522 in mouse liver lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 32 secs.

      The target band size is very close to IgG light chain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246522).

    • Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)
      Immunoprecipitation - Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)

      SIRT3 was immunoprecipitated from 0.35 mg rat liver lysate with ab246522 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab246522 at a 1/1000 dilution (0.45 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Rat liver lysate 10μg
      Lane 2: ab246522 IP in rat liver lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab246522 in rat liver lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 32 secs.

      The target band size is very close to IgG light chain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab246522).

    • Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)
      Anti-SIRT3 antibody [EPR23514-36] - BSA and Azide free (ab270523)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab270523 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab270523 は論文での使用が確認できていません。

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