Anti-SIRT2 抗体 [EP1668Y] (ab51023)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1668Y] to SIRT2
- Suitable for: Flow Cyt (Intra), WB, IP
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SIRT2 antibody [EP1668Y]
SIRT2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1668Y] to SIRT2 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IPmore details
適用なし: IHC-P -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human SIRT2 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: SKBR-3 cell lysate Flow Cyt (intra): HeLa cells IP: HeLa cell lysate
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Tissue culture supernatant -
ポリ/モノ
モノクローナル -
クローン名
EP1668Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab51023の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/30.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (3) |
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa).
|
IP | (1) |
1/50.
|
特記事項 |
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Flow Cyt (Intra)
1/30. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000. Detects a band of approximately 36 kDa (predicted molecular weight: 43 kDa). |
IP
1/50. |
ターゲット情報
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機能
NAD-dependent protein deacetylase, which deacetylates the 'Lys-40' of alpha-tubulin. Involved in the control of mitotic exit in the cell cycle, probably via its role in the regulation of cytoskeleton. -
組織特異性
Widely expressed. Highly expressed in heart, brain and skeletal muscle, while it is weakly expressed in placenta and lung. Down-regulated in many gliomas suggesting that it may act as a tumor suppressor gene in human gliomas possibly through the regulation of microtubule network. -
配列類似性
Belongs to the sirtuin family.
Contains 1 deacetylase sirtuin-type domain. -
発生段階
Peaks during mitosis. After mitosis, it is probably degraded by the 26S proteasome. -
翻訳後修飾
Phosphorylated at the G2/M transition of the cell cycle. -
細胞内局在
Cytoplasm > cytoskeleton. Colocalizes with microtubules. - Information by UniProt
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参照データベース
- Entrez Gene: 22933 Human
- Omim: 604480 Human
- SwissProt: Q8IXJ6 Human
- Unigene: 466693 Human
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別名
- FLJ35621 antibody
- FLJ37491 antibody
- NAD dependent deacetylase sirtuin 2 antibody
see all
画像
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Anti-SIRT2 antibody [EP1668Y] (ab51023) at 1/1000 dilution + SKBR-3 cell lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size: 43 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted? -
Purified ab51023 at 1/50 dilution (2µg) immunoprecipitating SIRT2 in HeLa whole cell lysate.
Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
Lane 2 (+): ab51023 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51023 in HeLa whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 36-43 kDa -
Intracellular Flow Cytometry analysis of permeabilized Hela cells using anti-SIR2/SIRT2 (red) (ab51023) or a rabbit IgG (negative) (green).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (13)
ab51023 は 13 報の論文で使用されています。
- Zhang X et al. Sirtuin 2 Alleviates Chronic Neuropathic Pain by Suppressing Ferroptosis in Rats. Front Pharmacol 13:827016 (2022). PubMed: 35401208
- Zhao Y et al. SIRT2-knockdown rescues GARS-induced Charcot-Marie-Tooth neuropathy. Aging Cell 20:e13391 (2021). PubMed: 34053152
- Ni Y et al. Silent information regulator 2 promotes clear cell renal cell carcinoma progression through deacetylation and small ubiquitin-related modifier 1 modification of glucose 6-phosphate dehydrogenase. Cancer Sci 112:4075-4086 (2021). PubMed: 34310804
- Cha Y et al. SIRT2 regulates mitochondrial dynamics and reprogramming via MEK1-ERK-DRP1 and AKT1-DRP1 axes. Cell Rep 37:110155 (2021). PubMed: 34965411
- Martínez-Gutiérrez A et al. Shikimic acid protects skin cells from UV-induced senescence through activation of the NAD+-dependent deacetylase SIRT1. Aging (Albany NY) 13:12308-12333 (2021). PubMed: 33901008