Application
Western blot
Sample
Monkey Tissue lysate - whole (Liver)
Loading amount
40 µg
Specification
Liver
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/250
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL+
Exposure
2 hour(s), 30 minute(s) and 0 second(s)
Bands
Specific: 75 kDa
Additional data
Abcam response
Thank you for your honest feedback. This antibody has not yet been verified to work with monkey samples, so it may very well be unsuitable in this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Oct 21 2009