Application
Western blot
Sample
Human Tissue lysate - whole (TUMOUR)
Gel Running Conditions
Reduced Non-Denaturing (Native) (4-20% gradient)
Loading amount
40 µg
Specification
TUMOUR
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/500
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (HRP)
Detection
Detection method
ECL+
Exposure
10 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Boh Sofela
Verified customer
投稿 Oct 27 2019