Anti-SIRP alpha 抗体 (ab8120)
Key features and details
- Rabbit polyclonal to SIRP alpha
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-SIRP alpha antibody
SIRP alpha 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SIRP alpha -
由来種
Rabbit -
特異性
Recognizes SIRPa1, 2 and 3. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Peptide corresponding to amino acids 487 to 503 ofhuman SIRPa1 (1). The sequences of immunogenicpeptide differ from those of mouse, rat and bovine by oneamino acid (1-7).
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ポジティブ・コントロール
- THP-1 whole cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading...
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精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8120の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
1/1000 - 1/2000. Detects a band of approximately 75-110 kDa.
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ICC/IF |
Use a concentration of 1 µg/ml.
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特記事項 |
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WB
1/1000 - 1/2000. Detects a band of approximately 75-110 kDa. |
ICC/IF
Use a concentration of 1 µg/ml. |
ターゲット情報
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機能
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
組織特異性
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
配列類似性
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
翻訳後修飾
N-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 140885 Human
- Omim: 602461 Human
- SwissProt: P78324 Human
- Unigene: 581021 Human
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別名
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
画像
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (10)
ab8120 は 10 報の論文で使用されています。
- Shui M et al. Anomalous Levels of CD47/Signal Regulatory Protein Alpha in the Hippocampus Lead to Excess Microglial Engulfment in Mouse Model of Perioperative Neurocognitive Disorders. Front Neurosci 16:788675 (2022). PubMed: 35360151
- Ding X et al. Loss of microglial SIRPa promotes synaptic pruning in preclinical models of neurodegeneration. Nat Commun 12:2030 (2021). PubMed: 33795678
- Liu Y et al. Podocyte-Released Migrasomes in Urine Serve as an Indicator for Early Podocyte Injury. Kidney Dis (Basel) 6:422-433 (2020). PubMed: 33313063
- Li L et al. Signal regulatory protein a protects podocytes through promoting autophagic activity. JCI Insight 5:N/A (2019). PubMed: 30888336
- Elberg G et al. Deletion of SIRPa (signal regulatory protein-a) promotes phagocytic clearance of myelin debris in Wallerian degeneration, axon regeneration, and recovery from nerve injury. J Neuroinflammation 16:277 (2019). PubMed: 31883525
- Whitaker AT et al. Smad4 regulates growth plate matrix production and chondrocyte polarity. Biol Open 6:358-364 (2017). PubMed: 28167493
- Almodóvar-García K et al. ANKRD1 acts as a transcriptional repressor of MMP13 via the AP-1 site. Mol Cell Biol 34:1500-11 (2014). PubMed: 24515436
- Irandoust M et al. Engagement of SIRPa inhibits growth and induces programmed cell death in acute myeloid leukemia cells. PLoS One 8:e52143 (2013). PubMed: 23320069
- Zhao XW et al. CD47-signal regulatory protein-a (SIRPa) interactions form a barrier for antibody-mediated tumor cell destruction. Proc Natl Acad Sci U S A 108:18342-7 (2011). PubMed: 22042861
- van Beek EM et al. Inhibitory regulation of osteoclast bone resorption by signal regulatory protein alpha. FASEB J 23:4081-90 (2009). PubMed: 19667119