Anti-SIL1 抗体 (ab5639)
Key features and details
- Goat polyclonal to SIL1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SIL1 antibody
SIL1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to SIL1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide:
ELLGSVNSLLKELR
, corresponding to C terminal amino acids 448-461 of Human SIL1. -
特記事項
SIL1 is a resident endoplasmic reticulum (ER) glycoprotein that interacts with the ATPase domain of BIP and enhances nucleotide exchange.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
一次抗体 備考
SIL1 is a resident endoplasmic reticulum (ER) glycoprotein that interacts with the ATPase domain of BIP and enhances nucleotide exchange. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5639の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 53 kDa).
A 1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 53 kDa). A 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5. -
組織特異性
Highly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain. -
関連疾患
Defects in SIL1 are a cause of Marinesco-Sjoegren syndrome (MSS) [MIM:248800]. MSS is an autosomal recessive multisystem disorder which is characterized by cerebellar ataxia due to cerebellar atrophy, with Purkinje and granule cell loss and myopathy featuring marked muscle replacement with fat and connective tissue. Other cardinal features include bilateral cataracts, hypergonadotrophic hypogonadism and mild to severe mental retardation. Skeletal abnormalities, short stature, dysarthria, strabismus and nystagmus are also frequent findings. Mutational inactivation of this protein may result in ER stress-induced cell death signaling or malfunctioning chaperone machineries that mishandle client proteins which are critical for the organs targeted in MSS. -
配列類似性
Belongs to the SIL1 family. -
発生段階
Expressed in fetal kidney, fetal lung, fetal liver and at low levels in fetal brain. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Endoplasmic reticulum lumen. - Information by UniProt
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参照データベース
- Entrez Gene: 64374 Human
- Omim: 608005 Human
- SwissProt: Q9H173 Human
- Unigene: 483521 Human
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別名
- BAP antibody
- BiP associated protein antibody
- BiP-associated protein antibody
see all
画像
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Western blot - Anti-SIL1 antibody (ab5639)
ab5639 staining (1
µ g/ml) of Human Kidney lysate (RIPA buffer, 30µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ab5639 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (6)
ab5639 は 6 報の論文で使用されています。
- Gatz C et al. Identification of Cellular Pathogenicity Markers for SIL1 Mutations Linked to Marinesco-Sjögren Syndrome. Front Neurol 10:562 (2019). PubMed: 31258504
- Xu H et al. SIL1 functions as an oncogene in glioma by AKT/mTOR signaling pathway. Onco Targets Ther 11:3775-3783 (2018). PubMed: 29997438
- Filézac de L'Etang A et al. Marinesco-Sjögren syndrome protein SIL1 regulates motor neuron subtype-selective ER stress in ALS. Nat Neurosci 18:227-38 (2015). IHC-FoFr, IHC-P ; Mouse, Human . PubMed: 25559081
- Anttonen AK et al. Novel SIL1 mutations and exclusion of functional candidate genes in Marinesco-Sjögren syndrome. Eur J Hum Genet 16:961-9 (2008). ICC/IF ; Mouse . PubMed: 18285827
- Liu Y et al. The orphan receptor tyrosine kinase Ror2 promotes osteoblast differentiation and enhances ex vivo bone formation. Mol Endocrinol 21:376-87 (2007). PubMed: 17095577
- Anttonen AK et al. The gene disrupted in Marinesco-Sjögren syndrome encodes SIL1, an HSPA5 cochaperone. Nat Genet 37:1309-11 (2005). IHC-Fr ; Human . PubMed: 16282978
