Anti-SIL1 抗体 (ab5639)

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Western blot - Anti-SIL1 antibody (ab5639)

    Key features and details

    • Goat polyclonal to SIL1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-SIL1 antibody
      SIL1 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to SIL1

    • 由来種

      Goat

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide:

      ELLGSVNSLLKELR

      , corresponding to C terminal amino acids 448-461 of Human SIL1.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項


      SIL1 is a resident endoplasmic reticulum (ER) glycoprotein that interacts with the ATPase domain of BIP and enhances nucleotide exchange.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% Tris buffered saline, 0.5% BSA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • 一次抗体 備考

      SIL1 is a resident endoplasmic reticulum (ER) glycoprotein that interacts with the ATPase domain of BIP and enhances nucleotide exchange.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    Our Abpromise guarantee covers the use of ab5639 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 55-60 kDa (predicted molecular weight: 53 kDa).

    A 1 hour primary incubation is recommended for this product.

    ターゲット情報

    • 機能

      Required for protein translocation and folding in the endoplasmic reticulum (ER). Functions as a nucleotide exchange factor for the ER lumenal chaperone HSPA5.

    • 組織特異性

      Highly expressed in tissues which produce large amounts of secreted proteins such as kidney, liver and placenta. Also expressed in colon, heart, lung, ovary, pancreas, peripheral leukocyte, prostate, spleen and thymus. Expressed at low levels throughout the brain.

    • 関連疾患

      Defects in SIL1 are a cause of Marinesco-Sjoegren syndrome (MSS) [MIM:248800]. MSS is an autosomal recessive multisystem disorder which is characterized by cerebellar ataxia due to cerebellar atrophy, with Purkinje and granule cell loss and myopathy featuring marked muscle replacement with fat and connective tissue. Other cardinal features include bilateral cataracts, hypergonadotrophic hypogonadism and mild to severe mental retardation. Skeletal abnormalities, short stature, dysarthria, strabismus and nystagmus are also frequent findings. Mutational inactivation of this protein may result in ER stress-induced cell death signaling or malfunctioning chaperone machineries that mishandle client proteins which are critical for the organs targeted in MSS.

    • 配列類似性

      Belongs to the SIL1 family.

    • 発生段階

      Expressed in fetal kidney, fetal lung, fetal liver and at low levels in fetal brain.

    • 翻訳後修飾

      N-glycosylated.

    • 細胞内局在

      Endoplasmic reticulum lumen.
    • Information by UniProt

    • 参照データベース

      • 別名

        • BAP antibody
        • BiP associated protein antibody
        • BiP-associated protein antibody
        • Endoplasmic reticulum chaperone SIL 1 antibody
        • Endoplasmic reticulum chaperone SIL1 antibody
        • MSS antibody
        • Nucleotide exchange factor SIL 1 antibody
        • Nucleotide exchange factor SIL1 antibody
        • SIL 1 antibody
        • sil1 antibody
        • SIL1 homolog antibody
        • SIL1 homolog endoplasmic reticulum chaperone (S. cerevisiae) antibody
        • SIL1 homolog endoplasmic reticulum chaperone antibody
        • SIL1_HUMAN antibody
        • UGL 5 antibody
        • ULG5 antibody
        see all

      画像

      • Western blot - Anti-SIL1 antibody (ab5639)
        Western blot - Anti-SIL1 antibody (ab5639)

        ab5639 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence.



        ab5639 staining (1µg/ml) of Human Kidney lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

      参考文献 (6)

      ab5639 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab5639 は 6 報の論文で使用されています。

      • Gatz C  et al. Identification of Cellular Pathogenicity Markers for SIL1 Mutations Linked to Marinesco-Sjögren Syndrome. Front Neurol 10:562 (2019). PubMed: 31258504
      • Xu H  et al. SIL1 functions as an oncogene in glioma by AKT/mTOR signaling pathway. Onco Targets Ther 11:3775-3783 (2018). PubMed: 29997438
      • Filézac de L'Etang A  et al. Marinesco-Sjögren syndrome protein SIL1 regulates motor neuron subtype-selective ER stress in ALS. Nat Neurosci 18:227-38 (2015). IHC-FoFr, IHC-P ; Mouse, Human . PubMed: 25559081
      • Anttonen AK  et al. Novel SIL1 mutations and exclusion of functional candidate genes in Marinesco-Sjögren syndrome. Eur J Hum Genet 16:961-9 (2008). ICC/IF ; Mouse . PubMed: 18285827
      • Liu Y  et al. The orphan receptor tyrosine kinase Ror2 promotes osteoblast differentiation and enhances ex vivo bone formation. Mol Endocrinol 21:376-87 (2007). PubMed: 17095577
      • Anttonen AK  et al. The gene disrupted in Marinesco-Sjögren syndrome encodes SIL1, an HSPA5 cochaperone. Nat Genet 37:1309-11 (2005). IHC-Fr ; Human . PubMed: 16282978

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