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Anti-SIGLEC H 抗体 [440c] (ab106174)

Reviews (1)Q&A (1)References (2)

Key features and details

  • Rat monoclonal [440c] to SIGLEC H
  • Suitable for: IHC-Fr, Flow Cyt, Inhibition Assay
  • Reacts with: Mouse
  • Isotype: IgG2b

製品の概要

  • 製品名

    Anti-SIGLEC H antibody [440c]
    SIGLEC H 一次抗体 製品一覧

  • 製品の詳細

    Rat monoclonal [440c] to SIGLEC H

  • 由来種

    Rat

  • アプリケーション

    適用あり: IHC-Fr, Flow Cyt, Inhibition Assaymore details

  • 種交差性

    交差種: Mouse

  • 免疫原

    Tissue, cells or virus corresponding to Mouse SIGLEC H. Purified bone-marrow derived mouse IPC

  • 特記事項


    Incubation of interferon producing cells with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing interferon producing cells depletion.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • バッファー

    Constituents: 0.1% BSA, PBS
  • Concentration information loading...

  • 精製度

    Protein G purified

  • 特記事項（精製）

    ab106174 was 0.2 µm filtered prior to formulation.

  • 一次抗体 備考

    Incubation of interferon producing cells with antibody 440c in vitro or administration of antibody 440c in vivo reduces secretion of IFN-alpha in response to CpG DNA without causing interferon producing cells depletion.

  • ポリ/モノ

    モノクローナル

  • クローン名

    440c

  • アイソタイプ

    IgG2b

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab106174の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Inhibition Assay
Use at an assay dependent concentration.

(For inhibition of IFN-alpha secretion dilutions have to be made according to the amounts of SIGLEC H to be inactivated).
特記事項
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt
Use at an assay dependent concentration.

ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Inhibition Assay
Use at an assay dependent concentration.

(For inhibition of IFN-alpha secretion dilutions have to be made according to the amounts of SIGLEC H to be inactivated).

ターゲット情報

  • 関連性

    SIGLEC H a cell-surface receptor molecule selectively expressed on murine natural interferon producing cells (IPC), also called mouse plasmacytoid dendritic cells (pDC). Siglec H is unique among Sialic acid-binding Ig-like lectins (Siglecs) proteins because it associates with the adaptor protein DAP12. DAP12 recognize certain viruses and CpG-DNA through TLR9, resulting in secretion of IFN-alpha, IL-12 and proinflammatory chemokines. Together these cytokines and chemokines recruite and activate NK cells and T cells as well as modulating the antigen presenting function of dendritic cells (DC). IPC themselves also function as antigen presenting cells that expand memory T cells and induce Th1 differentiation. Therefore IPC may provide a first line of host defense against viral infections by activating both innate and adaptive responses in vivo. The monoclonal antibody 440c exclusively recognizes mouse Siglec-H in all lymphoid organs under both normal and inflammatory conditions. Siglec-H is normal present in the T cell zone of lymph nodes and spleen.

  • 細胞内局在

    Cell Membrane

  • 参照データベース

    • 別名

      • sialic acid binding Ig like lectin H antibody
      • SIGLEC like protein antibody
      • Siglech antibody

    参考文献 (2)

    ab106174 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab106174 は 2 報の論文で使用されています。

    • Blasius AL  et al. Siglec-H is an IPC-specific receptor that modulates type I IFN secretion through DAP12. Blood 107:2474-6 (2006). PubMed: 16293595
    • Blasius A  et al. A cell-surface molecule selectively expressed on murine natural interferon-producing cells that blocks secretion of interferon-alpha. Blood 103:4201-6 (2004). PubMed: 14695235

    レビューと Q&A

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    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SIGLEC H antibody [440c]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Brain microglia)
    Permeabilization
    No
    Specification
    Brain microglia
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 09 2019

    Question

    phone call about suitability of ab106174 in IHC-P

    Read More
    Answer

    Merci de votre intérêt pour ab106174. Comme discuté au téléphone, cet anticorps n’a pas encore été testé IHC en coupes paraffine (IHC-P) à notre conaissance. Cependant, si vous souhaitez tester ab106174 IHC-P, il me sera possible de vous offrir une remise. Après soumission d’une Abreview avec vos résultats, une remise correspondant à la valeur d’achat de ab106174 pourra être utilisable lors d’une prochaine commande d’anticorps primaire.

    Etapes à suivre :

    1. Nous confirmer que vous souhaiteriez tester ab106174 en IHC-P afin de recevoir le code de remise correspondant. Ce code doit être édité avant l’achat de ab106174

    2. Acheter ab106174 par téléphone, fax ou internet (www.abcam.com)

    3. Le tester en IHC-P

    4. Nous faire part de vos résultats, positifs ou négatifs, grâce à notre système Abreview. Pour plus d’informations https://www.abcam.com/abreviews

    5. Après soumission de votre Abreview, appeler notre service clientèle afin de passer votre prochaine commande grâce au code de réduction. Ce code de réduction est utilisable 120 jours après son édition et utilisable lors de l’achat d’un autre anticorps primaire.
    Même si ab106174 est démontré ne pas fonctionner en IHC-P, après soumission de votre Abreview, vous recevrez quand même une remise sur l’achat de votre prochain anticorps.

    N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

    Termes et Conditions : www.abcam.com/collaborationdiscount.

    Read More

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