Anti-Sialoadhesin/CD169 抗体 [SP216] (ab183356)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP216] to Sialoadhesin/CD169
- Suitable for: Flow Cyt (Intra), IHC-P
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Sialoadhesin/CD169 antibody [SP216]
Sialoadhesin/CD169 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [SP216] to Sialoadhesin/CD169 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-P: Human bone marrow, HK lymphoma, squamous cell cervical carcinoma, squamous cell lung carcinoma and lymph node tissue. Flow Cyt (intra): Human PBMC treated with 20ng/ml IFN-gamma for 24hours cells
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A/G purified -
特記事項(精製)
Purified from TCS by protein A/G. -
ポリ/モノ
モノクローナル -
クローン名
SP216 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab183356の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/20.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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Flow Cyt (Intra)
1/20. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid (By similarity). Binds to SPN/CD43 on T-cells (By similarity). May play a role in hemopoiesis. -
組織特異性
Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis. -
配列類似性
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
細胞内局在
Secreted and Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 6614 Human
- Omim: 600751 Human
- SwissProt: Q9BZZ2 Human
- Unigene: 31869 Human
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別名
- CD169 antibody
- CD169 antigen antibody
- dJ1009E24.1 antibody
see all
画像
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Intracellular Flow Cytometry analysis of Human PBMC treated with 20ng/ml IFN-gamma for 24 hours cells labeling Sialoadhesin/CD169 with purified ab183356 at 1/20 dilution (47µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730). Unlabeled control - Unlabelled cells.
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Immunohistochemical analysis of formalin fixed, paraffin embedded human bone marrow tissue sections labeling Sialoadhesin/CD169 with ab183356 at a 1/100 dilution followed by rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins.
Positive staining on the macrophages in the human bone marrow, performed on a Leica Biosystems BOND® RX instrument.
The section was incubated with ab183356 for 30 mins at room temperature.
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Immunohistochemical analysis of formalin fixed, paraffin embedded human bone marrow tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.
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Immunohistochemical analysis of formalin fixed, paraffin embedded human HK lymphoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.
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Immunohistochemical analysis of formalin fixed, paraffin embedded human squamous cell cervical carcinoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.
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Immunohistochemical analysis of formalin fixed, paraffin embedded human squamous cell lung carcinoma tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.
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Immunohistochemical analysis of formalin fixed, paraffin embedded human lymph node tissue sections labeling Sialoadhesin/CD169 using ab183356 at a 1/100 dilution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab183356 は 1 報の論文で使用されています。
- Xiaocui L et al. CSF-1-induced DC-SIGN+ macrophages are present in the ovarian endometriosis. Reprod Biol Endocrinol 20:48 (2022). PubMed: 35260161