製品の概要
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製品名Anti-SIAH1 antibody
SIAH1 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to SIAH1
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由来種Goat
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アプリケーション適用あり: IHC-P, WB, IP, ELISAmore details
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種交差性交差種: Mouse, Rat, Human
交差が予測される動物種: Cow, Dog
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免疫原
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ポジティブ･コントロール
- Human liver lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 0.5mg/ml Tris, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
アプリケーション
Our Abpromise guarantee covers the use of ab2237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. PubMed: 17189520 1:300 donkey anti-goat was used for secondary antibody. |
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| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 28, 30 kDa (predicted molecular weight: 31, 35 kDa).Can be blocked with Human SIAH1 peptide (ab22876). | |
| IP | Use at an assay dependent concentration. | |
| ELISA | Use at an assay dependent concentration. |
ターゲット情報
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機能E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), the cell-surface receptor-type tyrosine kinase FLT3, the cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription regulation, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins.
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組織特異性Widely expressed at a low level. Down-regulated in advanced hepatocellular carcinomas.
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パスウェイProtein modification; protein ubiquitination.
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配列類似性Belongs to the SINA (Seven in absentia) family.
Contains 1 RING-type zinc finger.
Contains 1 SIAH-type zinc finger. -
ドメインThe RING-type zinc finger domain is essential for ubiquitin ligase activity.
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family. -
翻訳後修飾Phosphorylated on Ser-19 by ATM and ATR. This phosphorylation disrupts SIAH1 interaction with HIPK2, and subsequent proteasomal degradation of HIPK2.
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細胞内局在Cytoplasm. Nucleus. Predominantly cytoplasmic. Partially nuclear.
- Information by UniProt
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参照データベース
- Entrez Gene: 534655 Cow
- Entrez Gene: 6477 Human
- Entrez Gene: 20437 Mouse
- Entrez Gene: 140941 Rat
- Omim: 602212 Human
- SwissProt: Q8IUQ4 Human
- SwissProt: P61092 Mouse
- SwissProt: Q920M9 Rat
see all -
別名
- E3 ubiquitin-protein ligase SIAH1 antibody
- FLJ08065 antibody
- hSIAH1 antibody
see all
画像
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Western blot - Anti-SIAH1 antibody (ab2237)All lanes : Anti-SIAH1 antibody (ab2237) at 1 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Mouse liver lysate
Lane 4 : Rat liver lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 31, 35 kDa -
Western blot - Anti-SIAH1 antibody (ab2237)Anti-SIAH1 antibody (ab2237) at 1 µg/ml + Human liver lysate at 35 µg
Predicted band size: 31, 35 kDa
参考文献
This product has been referenced in:
- Van der Hoek KH et al. Viperin is an important host restriction factor in control of Zika virus infection. Sci Rep 7:4475 (2017). Read more (PubMed: 28667332) »
- Flores-Pérez A et al. Suppression of cell migration is promoted by miR-944 through targeting of SIAH1 and PTP4A1 in breast cancer cells. BMC Cancer 16:379 (2016). WB ; Human . Read more (PubMed: 27377268) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"