Key features and details
- Goat polyclonal to SHP2
- Suitable for: WB, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
精製度Immunogen affinity purified
特記事項（精製）Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab9214 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
1 hour primary incubation is recommended for this product.
|Flow Cyt||Use a concentration of 10 µg/ml.|
|ICC/IF||Use a concentration of 10 µg/ml.|
機能Acts downstream of various receptor and cytoplasmic protein tyrosine kinases to participate in the signal transduction from the cell surface to the nucleus.
組織特異性Widely expressed, with highest levels in heart, brain, and skeletal muscle.
関連疾患Defects in PTPN11 are the cause of LEOPARD syndrome type 1 (LEOPARD1) [MIM:151100]. It is an autosomal dominant disorder allelic with Noonan syndrome. The acronym LEOPARD stands for lentigines, electrocardiographic conduction abnormalities, ocular hypertelorism, pulmonic stenosis, abnormalities of genitalia, retardation of growth, and deafness.
Defects in PTPN11 are the cause of Noonan syndrome type 1 (NS1) [MIM:163950]. Noonan syndrome (NS) is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. Some patients with Noonan syndrome type 1 develop multiple giant cell lesions of the jaw or other bony or soft tissues, which are classified as pigmented villomoduolar synovitis (PVNS) when occurring in the jaw or joints. Note=Mutations in PTPN11 account for more than 50% of the cases. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS1 inheritance is autosomal dominant.
Defects in PTPN11 are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
Defects in PTPN11 are a cause of metachondromatosis (MC) [MIM:156250]. It is a skeletal disorder with radiologic fetarures of both multiple exostoses and Ollier disease, characterized by the presence of multiple enchondromas and osteochondroma-like lesions.
配列類似性Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain.
ドメインThe SH2 domains repress phosphatase activity. Binding of these domains to phosphotyrosine-containing proteins relieves this auto-inhibition, possibly by inducing a conformational change in the enzyme.
翻訳後修飾Phosphorylated on Tyr-546 and Tyr-584 upon receptor protein tyrosine kinase activation; which creates a binding site for GRB2 and other SH2-containing proteins.
- Information by UniProt
- BPTP3 antibody
- CFC antibody
- JMML antibody
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde-fixed, 0.15% triton-permeabilized HeLa cells staining SHP2 antibody with ab9214 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 2ug/ml. DAPI was used as a nuclear counterstain.
Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody at 2ug/ml
Flow cytometric analysis of paraformaldehyde-fixed, 0.5% Triton-permeabilized A431 cells (blue) staining SHP2 with ab9214 at 10µg/ml, followed by Alexa Fluor 488 secondary antibody at 1ug/ml. Primary incubation carried out for one hour.
IgG control: Unimmunized goat IgG followed by Alexa Fluor 488 secondary antibody (black).
Anti-SHP2 antibody (ab9214) at 2 µg/ml + Human muscle lysate at 35 µg
Predicted band size: 68 kDa
ab9214 staining (2
µg/ml) of Human Muscle lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. ab9214 staining (2µg/ml) of Human Muscle lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
ab9214 は 2 報の論文で使用されています。
- Liu HP et al. Association of supervillin with KIR2DL1 regulates the inhibitory signaling of natural killer cells. Cell Signal 23:487-96 (2011). WB ; Human . PubMed: 21070852
- Takagi K et al. Modulation of TNF-alpha gene expression by IFN-gamma and pamidronate in murine macrophages: regulation by STAT1-dependent pathways. J Immunol 174:1801-10 (2005). WB ; Mouse . PubMed: 15699106