Anti-SHP1 (phospho S591) 抗体 (ab41436)
Key features and details
- Rabbit polyclonal to SHP1 (phospho S591)
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SHP1 (phospho S591) antibody
SHP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SHP1 (phospho S591) -
由来種
Rabbit -
特異性
Phospho-Ser591-SHP1 specific. This antibody detects a 68 kDa band on SDS-PAGE immunoblots of human Jurkat and A431 cells treated with pervanadate, as well as A431 cells treated with EGF. -
アプリケーション
適用あり: ELISA, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide corresponding to Human SHP1 (phospho S591).
Database link: P29350 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
This antibody was cross-adsorbed to a non specific phospho-serine peptide then affinity purified using phospho-Ser591-SHP1 peptide (immunogen). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab41436の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use at an assay dependent concentration.
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WB |
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
ターゲット情報
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機能
Plays a key role in hematopoiesis. This PTPase activity may directly link growth factor receptors and other signaling proteins through protein-tyrosine phosphorylation. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. -
組織特異性
Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells. -
配列類似性
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 2 subfamily.
Contains 2 SH2 domains.
Contains 1 tyrosine-protein phosphatase domain. -
翻訳後修飾
Phosphorylated on serine and tyrosine residues. -
細胞内局在
Cytoplasm. Nucleus. In neurons, translocates into the nucleus after treatment with angiotensin II. - Information by UniProt
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参照データベース
- Entrez Gene: 5777 Human
- Entrez Gene: 15170 Mouse
- Entrez Gene: 116689 Rat
- Omim: 176883 Human
- SwissProt: P29350 Human
- SwissProt: P29351 Mouse
- SwissProt: P81718 Rat
- Unigene: 63489 Human
see all -
別名
- 70Z-SHP antibody
- EC 3.1.3.48 antibody
- HCP antibody
see all
画像
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Lanes 1-2 : anti-SHP1 (C-terminal)
Lanes 3-6 : Anti-SHP1 (phospho S591) antibody (ab41436) at 1/1000 dilution
Lanes 1 & 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min.
Lanes 2 & 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to lambda phosphatase before probing
Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with phospho-Ser591-SHP1 peptide
Lane 6 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with non specific phospho-serine peptide
Predicted band size: 68 kDa
Observed band size: 68 kDa
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab41436 は 1 報の論文で使用されています。
- Mkaddem SB et al. Lyn and Fyn function as molecular switches that control immunoreceptors to direct homeostasis or inflammation. Nat Commun 8:246 (2017). PubMed: 28811476