Sheep Anti-Rat IgG H&L (Biotin) (ab6851)
Key features and details
- Sheep Anti-Rat IgG H&L (Biotin)
- Conjugation: Biotin
- Host species: Sheep
- Suitable for: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Fr
製品の概要
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製品名
Sheep Anti-Rat IgG H&L (Biotin)
IgG 二次抗体 製品一覧 -
由来種
Sheep -
ターゲット生物種
Rat -
アプリケーション
適用あり: WB, ELISA, Immunomicroscopy, Dot blot, ICC/IF, IHC-P, IHC-Frmore details -
免疫原
Rat IgG whole molecule
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標識
Biotin
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate -
Concentration information loading... -
精製度
Affinity purified -
特記事項（精製）
Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC). Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule. -
特記事項（標識）
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities -
ポリ/モノ
ポリクローナル -
研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab6851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | ||
| ELISA | ||
| Immunomicroscopy | ||
| Dot blot | ||
| ICC/IF | ||
| IHC-P | ||
| IHC-Fr |
追加情報
immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency
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プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (3)
ab6851 は 3 報の論文で使用されています。
- Heuslein JL et al. Exposure of Endothelium to Biomimetic Flow Waveforms Yields Identification of miR-199a-5p as a Potent Regulator of Arteriogenesis. Mol Ther Nucleic Acids 12:829-844 (2018). PubMed: 30153567
- Heuslein JL et al. DNA Methyltransferase 1-Dependent DNA Hypermethylation Constrains Arteriogenesis by Augmenting Shear Stress Set Point. J Am Heart Assoc 6:N/A (2017). PubMed: 29191807
- Heuslein JL et al. Mechanisms of Amplified Arteriogenesis in Collateral Artery Segments Exposed to Reversed Flow Direction. Arterioscler Thromb Vasc Biol 35:2354-65 (2015). PubMed: 26338297
