Sheep Anti-Rabbit IgG H&L (FITC) (ab6791)

Submit a review Q&A (1)References (4)

Key features and details

  • Sheep Anti-Rabbit IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Sheep
  • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

製品の概要

  • 製品名

    Sheep Anti-Rabbit IgG H&L (FITC)
    IgG 二次抗体 製品一覧

  • 由来種

    Sheep

  • ターゲット生物種

    Rabbit

  • アプリケーション

    適用あり: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

  • 免疫原

    Rabbit whole molecule

  • 標識

    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 2.3 moles DTAF per mole of Rabbit IgG

  • 特記事項（標識）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • 追加情報
    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

    • プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (4)

    ab6791 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6791 は 4 報の論文で使用されています。

    • Lin JX  et al. Overexpression of IC53d promotes the proliferation of gastric cancer cells by activating the AKT/GSK3ß/cyclin D1 signaling pathway. Oncol Rep 41:2739-2752 (2019). PubMed: 30864700
    • Rocha-da-Silva L  et al. Expression of genome defence protein members in proliferating and quiescent rat male germ cells and the Nuage dynamics. PLoS One 14:e0217941 (2019). PubMed: 31181099
    • Meyer KB  et al. Identification of a Critical Window for Ganciclovir-Induced Disruption of Testicular Development in Rats. Toxicol Sci 162:488-498 (2018). PubMed: 29228316
    • Cantão IH  et al. An Initial Investigation of an Alternative Model to Study rat Primordial Germ Cell Epigenetic Reprogramming. Biol Proced Online 19:9 (2017). PubMed: 28785173

    レビューと Q&A

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    レビューを送る 質問を送る

    Question

    I am interested in using your sheep polyclonal anti rabbit Fitc ab6791 in immunocytochenistry but i want to know whether it has undergone any purification that would reduce cross reactivity to other species for multiple laballing. Particularly goat and donkey igG

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    Answer

    This antibody should not show cross-reactivity to goat or donkey IgG, since rabbits are substantially diverse to goats and donkeys. For instance, an anti-goat IgG would probably show cross-reactivity with sheep IgG and likewise for an anti-mouse IgG with rat IgG

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