Sheep Anti-Mouse IgG H&L (HRP) (ab6808)

Submit a review Q&A (1)References (26)

Key features and details

  • Sheep Anti-Mouse IgG H&L (HRP)
  • Conjugation: HRP
  • Host species: Sheep
  • Isotype: IgG
  • Suitable for: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB

製品の概要

  • 製品名

    Sheep Anti-Mouse IgG H&L (HRP)
    IgG 二次抗体 製品一覧

  • 由来種

    Sheep

  • ターゲット生物種

    Mouse

  • アプリケーション

    適用あり: ICC/IF, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details

  • 免疫原

    Mouse IgG whole molecule

  • 標識

    HRP

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    Preservative: 0.01% Gentamicin sulphate
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    Sheep Anti-Mouse IgG H&L was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500 - 1/2500.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent dilution.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Immunomicroscopy Use at an assay dependent concentration.
WB Use at an assay dependent dilution.

画像

  • Western blot - Sheep Anti-Mouse IgG H&L (HRP) (ab6808)
    Western blot - Sheep Anti-Mouse IgG H&L (HRP) (ab6808)
    Ab6808 was used at dilution 1/5000 with the primary antibody ab55120 in WB. See the review on ab55120.

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (26)

ab6808 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab6808 は 26 報の論文で使用されています。

  • Cowman S  et al. Decrease of Nibrin expression in chronic hypoxia is associated with hypoxia-induced chemoresistance in some brain tumour cells. BMC Cancer 19:300 (2019). PubMed: 30943920
  • Santini CC  et al. Global view of the RAF-MEK-ERK module and its immediate downstream effectors. Sci Rep 9:10865 (2019). PubMed: 31350469
  • Ye J  et al. Low-Concentration PTX And RSL3 Inhibits Tumor Cell Growth Synergistically By Inducing Ferroptosis In Mutant p53 Hypopharyngeal Squamous Carcinoma. Cancer Manag Res 11:9783-9792 (2019). PubMed: 31819616
  • Del Vecchio K  et al. A cationic, C-terminal patch and structural rearrangements in Ebola virus matrix VP40 protein control its interactions with phosphatidylserine. J Biol Chem 293:3335-3349 (2018). PubMed: 29348171
  • Zhu G  et al. Expression of TRPC5 is decreased in the sperm of patients with varicocele-associated asthenozoospermia. Biomed Rep 8:529-534 (2018). PubMed: 29774143
View all Publications for this product

レビューと Q&A

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Question

I am looking for a secondary antibody, a anti-mouse which is HRP conjugated. It has to be rat absorbed and suitable for Western blots. Can you help? Thankyou Rebecca Upton NHLI London

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Answer

We do not have such an antibody available at present, however we have sourced some and these will be added to our website catalogue by 25th May. You should be able to find them by browsing the secondary antibodies section under anti-mouse (choose from the list of conjugates to narrow the search). The product codes will be ab7061 and ab7068 (raised in donkey and goat, respectively).

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