Sheep Anti-Mouse IgG H&L (FITC) (ab6804)

Submit a review Q&A (6)

Key features and details

  • Sheep Anti-Mouse IgG H&L (FITC)
  • Conjugation: FITC. Ex: 493nm, Em: 528nm
  • Host species: Sheep
  • Suitable for: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA

製品の概要

  • 製品名

    Sheep Anti-Mouse IgG H&L (FITC)
    IgG 二次抗体 製品一覧

  • 由来種

    Sheep

  • ターゲット生物種

    Mouse

  • アプリケーション

    適用あり: Immunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISAmore details

  • 免疫原

    Mouse IgG whole molecule

  • 標識

    FITC. Ex: 493nm, Em: 528nm

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 1% BSA, 0.424% Potassium phosphate
  • Concentration information loading...

  • 精製度

    Affinity purified

  • 特記事項（精製）

    Diaminotriazinylaminofluorescein (DTAF) (Molecular Weight 530 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.3 moles DTAF per mole of Rabbit IgG

  • 特記事項（標識）

    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

  • ポリ/モノ

    ポリクローナル

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab6804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • 追加情報
    suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency

    • プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab6804 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6804 は論文での使用が確認できていません。

    レビューと Q&A

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    1-6 of 6 Abreviews or Q&A

    Question

    Hi again,

    I’ve now tested the new goat antimouse FITC secondary I received from Sigma and it worked very well with the vinculin primary and without any trouble. I still don’t know why the secondaries from abcam never worked, but it’s clear that the problem was with them. Could there have been some sort of issue with storage and shipping? How do I go about requesting a refund?

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    Answer

    Thank you for getting back to me and sharing your results.

    I am very pleased to hear that the new secondary antibody has worked well. This does indeed point to there having been a problem with the secondary antibody that you purchased from us as well as the replacement I sent you. I am not sure of the reason for this but I will be looking into it in more detail. I am very sorry for the inconvenience caused by these problems.

    I can arrange for a refund directly for you of the ab6804 if this is what you would like. This will be refunded directly to the account which paid for the original order.Alternatively, I can arrange for any antibody of your choice to be sent instead or raise a credit note which can be used for future purchases.

    Please let me know how you would like to proceed and I will arrange it for you.

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    Question

    Hi,

    Answers to the questionnaire attached.

    Thanks again.

    I'd appreciate any advice.

    Read More
    Answer

    Thank you for getting back to me. I am sorry for my late reply.

    I am really not sure where these experiments may be going wrong, with so many different conditions tried I really cannot explain why no staining has been observed in every case.I think I would try to find out a few fundamental things to try and rule outwhat may be contributing to the problems:

    1. Have you performed any other staining with a FITC conjugated secondary antibody which has worked? I would check that both of the secondary antibodies are working as expected by putting a little sample directly on a slide and taking an image.

    2. Do you have any anti-mouse IgG which you know have been working well with any other primary antibodies? Perhaps with a different conjugate? Could you try initially just to see if the two primary antibodies are detecting the expected protein by using this alternative secondary antibody?

    3. You mentioned that co-staining with Alexafluor has worked well. What primary and secondary antibodywere used for this staining? Which antibodies were applied in what order when the dual staining was performed?

    I look forward to receiving your reply.

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    Question

    Hi,

    Thank you for sending the replacement antibody. I’ve tried it and I still don’t get any staining. I just can’t figure out what’s wrong with what I’m doing. I’ve done this identical protocol before (but with different antibodies). I never had to do any sort of antigen retrieval or anything like that. I’ve tried with both primaries again and on 2 types of cells. I’m now going to attempt fixing with a fresh batch of PFA and fixing for a shorter period of time (10 minutes instead of 20). I’d be happy to have any other suggestions.

    Many thanks,

    Read More
    Answer

    Thank you for getting back to me.

    I am sorry to hear that the new secondary antibody has not made a difference in the results obtained. I would be happy to try to help in understanding why this might be happening.I think it would be useful if you could maybe fill in the questionnaire I have attached to this email. This information will allow me to see if there are any suggestions I would make in altering the protocol used.

    I think reducing the fixation time and using fresh PFA is a good start and I'll let you know if I have any further suggestions once I have received the completed questionnaire.

    I look forward to receiving your reply.

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    Question

    Many thanks. Please send the Goat secondary (ab97239). Could you make sure it gets sent right away? I'm out of town next week and I'd like to be able to try it before I leave so I can let my student work with it while I'm gone.

    All the best,

    Read More
    Answer

    Thank you for confirming that for me.

    I have arranged for the replacement to be sent to you. This is on the order number xxxxx(Purchase order number FOCR xxxxxx). This should be with you tomorrow. Please let me know if you have any problems receiving it.

    If you continue to have problems in the staining please do also let me know.

    Until then, I wish you all the best with your experiments.

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    Question

    Hi,

    The alternative primary antibody was ab16821 (a mouse monoclonal to alpha v integrin). I ordered this antibody on 16 July, but it only arrived a few days ago.

    Read More
    Answer

    Thank you for confirming that.

    I can send you a new secondary antibody to try if you would like. I would suggest the following would be suitable:

    Goat polyclonal Secondary Antibody to Mouse IgG1 - heavy chain (FITC) (ab97239)

    Sheep polyclonal Secondary Antibody to Mouse IgG - H&L (FITC) (ab102457)

    If you let me know which one you would like to try (or a different one altogether if you prefer). I can then arrange for it to be sent to you, free of charge.

    I look forward to receiving your reply.

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    Question

    Hi again,

    We’ve tried the staining again today with the alpha V integrin primary antibody that we ordered with the same order on 16 July. Again, there has been no staining. I’m guessing this means that the secondary antibody is faulty. Could you recommend an alternative, also conjugated to FITC and ship it to me as soon as possible? This has been holding up my work for weeks. I’d be happy to have one raised in Goat if the choices are more limited for those raised in Sheep.

    Many thanks,

    Read More
    Answer

    Thank you for getting back to me with that information.

    I am sorry but I am a little confused with what has been tested? Which alternative antibody was tried with the secondary antibody which again did not work? On the orderof the 16th of July I only have the ab18058 and ab6804. Do you know the catalogue number of the antibody used?

    I will try to resolve this situation as soon as possible. I am sorry for the inconvenience this problem has been causing you.

    I look forward to receiving your reply.

    Read More

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