Anti-SHB 抗体 [EPR7976] - BSA and Azide free (ab248341)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7976] to SHB - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SHB antibody [EPR7976] - BSA and Azide free
SHB 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR7976] to SHB - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: Flow Cyt,ICC/IF,IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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特記事項
ab248341 is the carrier-free version of ab129190.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
解離定数(KD 値)
KD = 1.21 x 10 -11 M Learn more about KD -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR7976 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Conjugation kits
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab248341の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa. |
ターゲット情報
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機能
Adapter protein which regulates several signal transduction cascades by linking activated receptors to downstream signaling components. May play a role in angiogenesis by regulating FGFR1, VEGFR2 and PDGFR signaling. May also play a role in T-cell antigen receptor/TCR signaling, interleukin-2 signaling, apoptosis and neuronal cells differentiation by mediating basic-FGF and NGF-induced signaling cascades. May also regulate IRS1 and IRS2 signaling in insulin-producing cells. -
組織特異性
Widely expressed. -
配列類似性
Contains 1 SH2 domain. -
ドメイン
The SH2 domain preferentially binds phosphopeptides with the consensus sequence Y-[TVI]-X-L and mediates interaction with PDGFRA, PDGFRB, FGRFR1, IL2RB, IL2RG, CD3Z and CRK/CrKII. -
翻訳後修飾
Phosphorylated upon PDGFRA, PDGFRB, TCR, IL2 receptor, FGFR1 or VEGFR2 activation. -
細胞内局在
Cytoplasm. Cell membrane. Associates with membrane lipid rafts upon TCR stimulation. - Information by UniProt
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参照データベース
- Entrez Gene: 6461 Human
- Entrez Gene: 230126 Mouse
- Entrez Gene: 362513 Rat
- Omim: 600314 Human
- SwissProt: Q15464 Human
- SwissProt: Q6PD21 Mouse
- Unigene: 521482 Human
- Unigene: 251716 Mouse
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別名
- bA3J10.2 antibody
- OTTHUMP00000021397 antibody
- RP11 3J10.8 antibody
see all
画像
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All lanes : Anti-SHB antibody [EPR7976] (ab129190) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : K562 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDaThis data was developed using ab129190, the same antibody clone in a different buffer formulation.
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This data was developed using ab129190, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab248341 は論文での使用が確認できていません。