Anti-SH3BP2 (phospho S427) 抗体 (ab2176)
製品の概要
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製品名
Anti-SH3BP2 (phospho S427) antibody
SH3BP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SH3BP2 (phospho S427)This product is a fast track antibody. It has been affinity purified and shows high titre values against the immunizing peptide by ELISA. Read the terms of use »
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由来種
Rabbit -
種交差性
交差が予測される動物種: Human
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免疫原
Synthetic phospho peptide: SFRSFSpFEKPRQ, corresponding to amino acids 422-433 of Human SH3BP2.
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特記事項
Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase).
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Affinity purified chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. -
一次抗体 備考
Phosphorylation of SH3BP2 occurs on Ser 427 for activation. SH3BP2 mediates interactions of huntingtin and MLK2 (mixed lineage kinase). -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). Fast track terms of use
| アプリケーション | Abreviews | 特記事項 |
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| WB | Use at an assay dependent concentration. Predicted molecular weight: 62 kDa. Reacts in Western blotting with a band at about 60kD, that probably corresponds to SH3BP1 (Not tested for phospho specificity by Western). |
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| ELISA | Use at an assay dependent concentration. Tested in peptide ELISA against 0.1 ug of the immunizing peptide. |
ターゲット情報
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機能
Binds differentially to the SH3 domains of certain proteins of signal transduction pathways. Binds to phosphatidylinositols; linking the hemopoietic tyrosine kinase fes to the cytoplasmic membrane in a phosphorylation dependent mechanism. -
組織特異性
Expressed in a variety of tissues including lung, liver, skeletal muscle, kidney and pancreas. -
関連疾患
Defects in SH3BP2 are the cause of cherubism (CRBM) [MIM:118400]. CRBM is an autosomal dominant inherited syndrome characterized by excessive bone degradation of the upper and lower jaws, which often begins around three years of age. It is followed by development of fibrous tissue masses, which causes a characteristic facial swelling. -
配列類似性
Contains 1 PH domain.
Contains 1 SH2 domain. - Information by UniProt
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参照データベース
- Entrez Gene: 6452 Human
- Omim: 602104 Human
- SwissProt: P78314 Human
- Unigene: 167679 Human
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別名
- 3BP-2 antibody
- 3BP2 antibody
- 3BP2_HUMAN antibody
see all
画像
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Western blot - Anti-SH3BP2 (phospho S427) antibody (ab2176)Western blot analysis is shown using ab2176 to detect endogenous protein present in unstimulated human whole cell lysates. The band as indicated by the arrowheads is evident in both M059 cells (panel A) and PC-3 cells (panel B). Comparison to a molecular weight marker indicates a band of ~60 kDa that probably corresponds to human SH3BP2 protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using standard techniques.
プロトコール
データシートおよび資料
参考文献
ab2176 has not yet been referenced specifically in any publications.
