Anti-SH3BGRL3 抗体 (ab122138)
Key features and details
- Rabbit polyclonal to SH3BGRL3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SH3BGRL3 antibody -
製品の詳細
Rabbit polyclonal to SH3BGRL3 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
antigen sequence: SEVTRILDGK RIQYQLVDIS QDNALRDEMR ALAGNPKATP PQIVNGDQYC GDYELFVEAV EQNTLQEFLK LA, corresponding to amino acids 22-93 of Human SH3BGRL3
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ポジティブ・コントロール
- Human smooth muscle tissue; RT4 cell lysates and Human liver and tonsil tissue lysates; U2OS cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122138の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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ICC/IF |
Use a concentration of 1 - 4 µg/ml. Fixed/permeabilized with PFA/Triton X-100
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特記事項 |
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IHC-P
1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
ICC/IF
Use a concentration of 1 - 4 µg/ml. Fixed/permeabilized with PFA/Triton X-100 |
ターゲット情報
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機能
Could act as a modulator of glutaredoxin biological activity. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the SH3BGR family.
Contains 1 glutaredoxin domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 83442 Human
- SwissProt: Q9H299 Human
- Unigene: 109051 Human
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別名
- epididymis secretory protein Li 297 antibody
- HEL-S-297 antibody
- SH3 domain binding glutamic acid rich protein like 3 antibody
see all
画像
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All lanes : Anti-SH3BGRL3 antibody (ab122138) at 1/250 dilution
Lane 1 : RT4 cell lysates
Lane 2 : U251MG cell lysates
Lane 3 : Human plasma
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate -
ab122138, at 1/600 dilution, staining SH3BGRL3 in Human smooth muscle tissue by Immunohistochemistry using paraffin-embedded tissue.
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ab122138 at 4 µg/ml staining SH3BGRL3 in PFA/Triton X-100 fixed/permeabilized U2OS cells by Immunofluorescence. Antibody staining is shown in green
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab122138 は論文での使用が確認できていません。