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リコンビナントRabMAb

Anti-SH2D1A/SAP 抗体 [EPR3168] (ab109120)

Submit a review Q&A (2)
Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
  • Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
  • Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
  • Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3168] to SH2D1A/SAP
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-SH2D1A/SAP antibody [EPR3168]
    SH2D1A/SAP 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR3168] to SH2D1A/SAP

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details
    適用なし: Flow Cyt,ICC/IF or IHC-P

  • 種交差性

    交差種: Mouse, Rat, Human

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Molt-4, Jurkat, PC-12 and K562 cell lysates and mouse brain tissue lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.

  • バッファー

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • 精製度

    Protein A purified

  • ポリ/モノ

    モノクローナル

  • クローン名

    EPR3168

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab109120の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
特記事項
WB
1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 14 kDa).
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

ターゲット情報

  • 機能

    Inhibitor of the SLAM self-association. Acts by blocking recruitment of the SH2-domain-containing signal-transduction molecule SHP-2 to a docking site in the SLAM cytoplasmic region. Mediates interaction between FYN and SLAMF1. May also regulate the activity of the neurotrophin receptors NTRK1, NTRK2 and NTRK3.

  • 組織特異性

    Expressed at a high level in thymus and lung, with a lower level of expression in spleen and liver. Expressed in peripheral blood leukocytes, including T lymphocytes. Tends to be expressed at lower levels in peripheral blood leukocytes in patients with rheumatoid arthritis.

  • 関連疾患

    Defects in SH2D1A are a cause of lymphoproliferative syndrome X-linked type 1 (XLP1) [MIM:308240]; also known as X-linked lymphoproliferative disease (XLPD) or Duncan disease. XLP is a rare immunodeficiency characterized by extreme susceptibility to infection with Epstein-Barr virus (EBV). Symptoms include severe or fatal mononucleosis, acquired hypogammaglobulinemia, pancytopenia and malignant lymphoma.

  • 配列類似性

    Contains 1 SH2 domain.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • DSHP antibody
    • Duncan disease SH2 protein antibody
    • Duncan disease SH2-protein antibody
    • EBVS antibody
    • IMD5 antibody
    • LYP antibody
    • MTCP1 antibody
    • SAP antibody
    • SAP/SH2D1A antibody
    • SH2 domain containing 1A antibody
    • SH2 domain protein 1A antibody
    • SH2 domain-containing protein 1A antibody
    • SH21A_HUMAN antibody
    • SH2D1A antibody
    • Signaling lymphocyte activation molecule associated protein antibody
    • Signaling lymphocytic activation molecule-associated protein antibody
    • SLAM associated protein antibody
    • SLAM associated protein/SH2 domain protein 1A antibody
    • SLAM-associated protein antibody
    • T cell signal transduction molecule SAP antibody
    • T-cell signal transduction molecule SAP antibody
    • XLP antibody
    • XLPD antibody
    see all

画像

  • Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution (purified)

    Lane 1 : PC-12 whole cell lysate
    Lane 2 : Mouse brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 14 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/2000 dilution (purified)

    Lane 1 : Molt-4 whole cell lysate
    Lane 2 : Jurkat whole cell lysate
    Lane 3 : K562 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 14 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?



    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    Western blot - Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    All lanes : Anti-SH2D1A/SAP antibody [EPR3168] (ab109120) at 1/1000 dilution (unpurified)

    Lane 1 : MOLT-4 cell line lysate
    Lane 2 : Jurkat cell line lysate
    Lane 3 : K562 cell line lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 14 kDa

  • Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)
    Anti-SH2D1A/SAP antibody [EPR3168] (ab109120)

参考文献 (0)

ab109120 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109120 は論文での使用が確認できていません。

レビューと Q&A

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レビューを送る 質問を送る

1-2 of 2 Abreviews or Q&A

Question

Can you please provide a representative plot for the flow data for this Ab (ab109120).

Thank you.

Read More
Answer

I now have further data to share with you in regards to the Anti-SH2D1A/SAP antibody [EPR3168] (ab109120).

I have attached the histogram produced when using the antibody to detect Molt-4 cells.

I hope this information has been of help. If you have any further questions, please do not hesitate to contact usagain.

Read More

Question

Recently I've ordered product Anti-SH2D1A/SAP (ab109120).
Can you tell me the concentration of this antibody? I could not find it anywhere...Thank you in advance,

Read More
Answer

Thank you for your inquiry.
The concentration for ab109120, lot GR61496-3, is 0.781 mg/mL.
I hope this information helps. Please contact us with any other questions.

Read More

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