Anti-SFRP2 抗体 - C-terminal (ab137560)
Key features and details
- Rabbit polyclonal to SFRP2 - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SFRP2 antibody - C-terminal
SFRP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SFRP2 - C-terminal -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog, Zebrafish -
免疫原
Synthetic peptide, corresponding to a region within C terminal amino acids 231-295 of Human SFRP2.
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ポジティブ・コントロール
- 293T, A431, H1299, HeLa, HepG2, Molt 4 and Raji cell lysates; A431 cells; MCF7 xenograft
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab137560の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol
using 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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特記事項 |
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ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
ターゲット情報
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機能
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. -
組織特異性
Expressed in adipose tissue, heart, brain, skeletal muscle, pancreas, thymus, prostate, testis, ovary, small intestine and colon. Highest levels in adipose tissue, small intestine and colon. -
配列類似性
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
ドメイン
The FZ domain is involved in binding with Wnt ligands. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 475471 Dog
- Entrez Gene: 6423 Human
- Entrez Gene: 20319 Mouse
- Entrez Gene: 310552 Rat
- Entrez Gene: 566878 Zebrafish
- Omim: 604157 Human
- SwissProt: Q863H1 Dog
- SwissProt: Q96HF1 Human
see all -
別名
- AI851596 antibody
- Frizzled related protein 2 antibody
- FRP 2 antibody
see all
画像
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All lanes : Anti-SFRP2 antibody - C-terminal (ab137560) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : Hela whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling SFRP2 with ab137560 at 1/200 dilution. Lower image is merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded MCF7 xenograft labelling SFRP2 with ab137560 at 1/500 dilution.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab137560 は 10 報の論文で使用されています。
- Kasashima H et al. Stromal SOX2 Upregulation Promotes Tumorigenesis through the Generation of a SFRP1/2-Expressing Cancer-Associated Fibroblast Population. Dev Cell 56:95-110.e10 (2021). PubMed: 33207226
- Wu Q et al. Downregulation of SFRP2 facilitates cancer stemness and radioresistance of glioma cells via activating Wnt/β-catenin signaling. PLoS One 16:e0260864 (2021). PubMed: 34852024
- Montagner M et al. Crosstalk with lung epithelial cells regulates Sfrp2-mediated latency in breast cancer dissemination. Nat Cell Biol 22:289-296 (2020). PubMed: 32094692
- Fane ME et al. sFRP2 Supersedes VEGF as an Age-related Driver of Angiogenesis in Melanoma, Affecting Response to Anti-VEGF Therapy in Older Patients. Clin Cancer Res 26:5709-5719 (2020). PubMed: 33097493
- Hu S et al. Dermal exosomes containing miR-218-5p promote hair regeneration by regulating ß-catenin signaling. Sci Adv 6:eaba1685 (2020). PubMed: 32832660
- Shi J et al. Overexpression of LINC00261 inhibits non-small cell lung cancer cells progression by interacting with miR-522-3p and suppressing Wnt signaling. J Cell Biochem 120:18378-18387 (2019). PubMed: 31190356
- Liang J et al. Gossypol Promotes Wnt/ß-Catenin Signaling through WIF1 in Ovariectomy-Induced Osteoporosis. Biomed Res Int 2019:8745487 (2019). PubMed: 31139657
- Liu S et al. miR-221/222 activate the Wnt/ß-catenin signaling to promote triple-negative breast cancer. J Mol Cell Biol 10:302-315 (2018). PubMed: 30053090
- Cai J et al. Simultaneous overactivation of Wnt/ß-catenin and TGFß signalling by miR-128-3p confers chemoresistance-associated metastasis in NSCLC. Nat Commun 8:15870 (2017). PubMed: 28627514
- Kaur A et al. sFRP2 in the aged microenvironment drives melanoma metastasis and therapy resistance. Nature 532:250-4 (2016). ELISA . PubMed: 27042933