リコンビナントRabMAb

Anti-SFRP1 抗体 [EPR23092-253] - BSA and Azide free (ab269949)

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Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
  • Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
  • Flow Cytometry - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
  • Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23092-253] to SFRP1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP
  • Reacts with: Mouse, Rat, Human

製品の概要

  • 製品名

    Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free
    SFRP1 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR23092-253] to SFRP1 - BSA and Azide free

  • 由来種

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    See all applications and species data

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • WB: Recombinant human sFRP-1 protein. Human breast, kidney and heart tissue lysate. SK-MEL-28 whole cell lysate. Mouse and rat kidney tissue lysate. NIH/3T3 whole cell lysate. Flow Cyt: BFA treated SK-MEL-28 cells. IP: Human and mouse kidney tissue lysate.

  • 特記事項

    ab269949 is the carrier-free version of ab267466. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab269949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
Flow Cyt
Human
IP
Human
アプリケーション Abreviews 特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
特記事項
WB
Use at an assay dependent concentration. Predicted molecular weight: 35 kDa.
Flow Cyt
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
  • 追加情報
    Is unsuitable for ICC/IF or IHC-P.

    • ターゲット情報

    • 機能

      Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.

    • 組織特異性

      Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.

    • 配列類似性

      Belongs to the secreted frizzled-related protein (sFRP) family.
      Contains 1 FZ (frizzled) domain.
      Contains 1 NTR domain.

    • ドメイン

      The FZ domain is involved in binding with Wnt ligands.

    • 細胞内局在

      Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • Frizzled related protein 1 antibody
      • FRP 1 antibody
      • FRP antibody
      • FRP-1 antibody
      • FRP1 antibody
      • FrzA antibody
      • SARP 2 antibody
      • SARP-2 antibody
      • SARP2 antibody
      • Secreted apoptosis related protein 2 antibody
      • Secreted apoptosis-related protein 2 antibody
      • Secreted frizzled related protein 1 antibody
      • Secreted frizzled related protein antibody
      • Secreted frizzled-related protein 1 antibody
      • SFRP 1 antibody
      • sFRP-1 antibody
      • SFRP1 antibody
      • SFRP1_HUMAN antibody
      see all

    画像

    • Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
      Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)

      SFRP1 was immunoprecipitated from 0.35 mg human kidney lysate 10µg with ab267466 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: Human kidney lysate 10µg

      Lane 2: ab267466 IP in human kidney lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267466 in human kidney lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 30 seconds.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267466).

    • Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
      Immunoprecipitation - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)

      SFRP1 was immunoprecipitated from 0.35 mg mouse kidney lysate 10ug with ab267466 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

      Lane 1: Mouse kidney lysate 10µg

      Lane 2: ab267466 IP in mouse kidney lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267466 in mouse kidney lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 mins.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267466).

    • Flow Cytometry - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
      Flow Cytometry - Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)

      Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, Left) treated with 5μg/ml BFA for 2h / SK-MEL-28 (Human malignant melanoma, Right) treated with 5μg/ml BFA for 2h labeling SFRP1 with ab267466 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.

      Negative control: MCF7 (PMID: 14968126).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab267466).

    • Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)
      Anti-SFRP1 antibody [EPR23092-253] - BSA and Azide free (ab269949)

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab269949 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab269949 は論文での使用が確認できていません。

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