Anti-SFRP1 抗体 [EPR23092-253] (ab267466)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23092-253] to SFRP1
- Suitable for: WB, Flow Cyt (Intra), IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-SFRP1 antibody [EPR23092-253]
SFRP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23092-253] to SFRP1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, Flow Cyt (Intra), IPmore details
適用なし: ICC/IF or IHC-P -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Recombinant human sFRP-1 protein. Human breast, kidney and heart tissue lysate. SK-MEL-28 whole cell lysate. Mouse and rat kidney tissue lysate. NIH/3T3 whole cell lysate. Flow Cyt (intra): BFA treated SK-MEL-28 cells. IP: Human and mouse kidney tissue lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23092-253 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab267466の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Predicted molecular weight: 35 kDa.
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Flow Cyt (Intra) |
1/500.
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IP |
1/30.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 35 kDa. |
Flow Cyt (Intra)
1/500. |
IP
1/30. |
ターゲット情報
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機能
Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription. -
組織特異性
Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. -
配列類似性
Belongs to the secreted frizzled-related protein (sFRP) family.
Contains 1 FZ (frizzled) domain.
Contains 1 NTR domain. -
ドメイン
The FZ domain is involved in binding with Wnt ligands. -
細胞内局在
Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding. - Information by UniProt
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参照データベース
- Entrez Gene: 6422 Human
- Entrez Gene: 20377 Mouse
- Entrez Gene: 84402 Rat
- Omim: 604156 Human
- SwissProt: Q8N474 Human
- SwissProt: Q8C4U3 Mouse
- SwissProt: Q9R168 Rat
- Unigene: 213424 Human
see all -
別名
- Frizzled related protein 1 antibody
- FRP 1 antibody
- FRP antibody
see all
画像
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All lanes : Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/1000 dilution
Lane 1 : Rat kidney lysate at 20 µg
Lane 2 : NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22927647, 11741940).
Exposure time: 92 seconds.
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All lanes : Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/1000 dilution
Lane 1 : Human breast lysate
Lane 2 : Human kidney lysate
Lane 3 : Human heart lysate
Lane 4 : SK-MEL-28 (human malignant melanoma) whole cell lysate
Lane 5 : MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate
Lane 6 : Mouse kidney lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 22927647, 11741940).
Negative control: MCF7 (PMID:15289865).
Exposure time: 92 seconds.
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Anti-SFRP1 antibody [EPR23092-253] (ab267466) at 1/5000 dilution + Recombinant human sFRP-1 protein, 20 ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 35 kDa
Observed band size: 35 kDaBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 114 seconds.
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SFRP1 was immunoprecipitated from 0.35 mg mouse kidney lysate with ab267466 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Mouse kidney lysate 10µg
Lane 2: ab267466 IP in mouse kidney lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267466 in mouse kidney lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
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SFRP1 was immunoprecipitated from 0.35 mg human kidney lysate with ab267466 at 1/30 dilution (2µg in 0.35 mg lysates). Western blot was performed on the immunoprecipitate using ab267466 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.
Lane 1: Human kidney lysate 10µg
Lane 2: ab267466 IP in human kidney lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab267466 in human kidney lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
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Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MCF7 (Human breast adenocarcinoma epithelial cell, Left) treated with 5µg/ml BFA for 2h / SK-MEL-28 (Human malignant melanoma, Right) treated with 5µg/ml BFA for 2h labeling SFRP1 with ab267466 at 1/500 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 was used as the secondary antibody.
Negative control: MCF7 (PMID: 14968126).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (2)
ab267466 は 2 報の論文で使用されています。
- Hou Y et al. Oncogenic miR-27a delivered by exosomes binds to SFRP1 and promotes angiogenesis in renal clear cell carcinoma. Mol Ther Nucleic Acids 24:92-103 (2021). PubMed: 33738141
- Liang L et al. Melatonin pretreatment alleviates the long-term synaptic toxicity and dysmyelination induced by neonatal Sevoflurane exposure via MT1 receptor-mediated Wnt signaling modulation. J Pineal Res 71:e12771 (2021). PubMed: 34585785