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    sfrp1-antibody-ab4193.pdf

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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome
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Anti-SFRP1 抗体 (ab4193)

  • Datasheet
  • SDS
Reviews (4)Q&A (15)References (38)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-SFRP1 antibody (ab4193)
  • Immunocytochemistry/ Immunofluorescence - Anti-SFRP1 antibody (ab4193)

Key features and details

  • Rabbit polyclonal to SFRP1
  • Suitable for: ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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関連製品

製品の概要

  • 製品名

    Anti-SFRP1 antibody
    SFRP1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to SFRP1
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: ICC/IF, WBmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide:

    IGPYQSGRFYTKC

    , corresponding to amino acids 42 - 53 of Human SFRP1.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Preservative: 0.01% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • 特記事項(精製)

    This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Gut specification

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human SFRP1 protein (ab64445)

アプリケーション

Our Abpromise guarantee covers the use of ab4193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB 1/230. Predicted molecular weight: 37 kDa.

ターゲット情報

  • 機能

    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
  • 組織特異性

    Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
  • 配列類似性

    Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • ドメイン

    The FZ domain is involved in binding with Wnt ligands.
  • 細胞内局在

    Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
  • Target information above from: UniProt accession Q8N474 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 6422 Human
    • Omim: 604156 Human
    • SwissProt: Q8N474 Human
    • Unigene: 213424 Human
    • 別名

      • Frizzled related protein 1 antibody
      • FRP 1 antibody
      • FRP antibody
      • FRP-1 antibody
      • FRP1 antibody
      • FrzA antibody
      • SARP 2 antibody
      • SARP-2 antibody
      • SARP2 antibody
      • Secreted apoptosis related protein 2 antibody
      • Secreted apoptosis-related protein 2 antibody
      • Secreted frizzled related protein 1 antibody
      • Secreted frizzled related protein antibody
      • Secreted frizzled-related protein 1 antibody
      • SFRP 1 antibody
      • sFRP-1 antibody
      • SFRP1 antibody
      • SFRP1_HUMAN antibody
      see all

    画像

    • Western blot - Anti-SFRP1 antibody (ab4193)
      Western blot - Anti-SFRP1 antibody (ab4193)This image is courtesy of Becky Mercer, University of Columbia

      Western blot using ab4193 at 1/230 (o/n 4°C) on lysates from cultured small airway epithelial cells.

      Expected band size: 37kD.

      Western blot using ab4193 at 1/230 (o/n 4°C) on lysates from cultured small airway epithelial cells.

    • Immunocytochemistry/ Immunofluorescence - Anti-SFRP1 antibody (ab4193)
      Immunocytochemistry/ Immunofluorescence - Anti-SFRP1 antibody (ab4193)
      ICC/IF image of ab4193 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4193, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    プロトコール

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (38)

    ab4193 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4193 は 38 報の論文で使用されています。

    • Zhou J  et al. Dynamic decreased expression and hypermethylation of secreted frizzled-related protein 1 and 4 over the course of pulmonary fibrosis in mice. Life Sci 218:241-252 (2019). PubMed: 30586565
    • Robson JP  et al. Identification of CD24 as a marker of Patched1 deleted medulloblastoma-initiating neural progenitor cells. PLoS One 14:e0210665 (2019). PubMed: 30657775
    • Hawkshaw NJ  et al. Deciphering the molecular morphology of the human hair cycle: Wnt signalling during the telogen-anagen transformation. Br J Dermatol N/A:N/A (2019). PubMed: 31314901
    • Esteve P  et al. Elevated levels of Secreted-Frizzled-Related-Protein 1 contribute to Alzheimer's disease pathogenesis. Nat Neurosci 22:1258-1268 (2019). PubMed: 31308530
    • Gopinathan G  et al. The Wnt Antagonist SFRP1: A Key Regulator of Periodontal Mineral Homeostasis. Stem Cells Dev 28:1004-1014 (2019). PubMed: 31215318
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 19 Abreviews or Q&A

    Question

    I am using SFRP 1 ab4193 in medulloblastomas. but i hav used the following dilutions and controls. However, it is not coming positive in any of the controls or cases. 1:25, 1:50, 1:100, 1:200, 1:400, 1:600 in heart, fetal kidney, hypertrophic scar, normal breast, medulloblastoma cases with citrate antigen retreival at ph 6, for 20 min, in microwave also with quanto kit, and overnight incubation at 4degree celcius Please let me know if i am doin anything wrong and also can u suggest some control, antigen retreival method and localization of antibody(cytoplasmic, membranous or nuclear).

    Read More

    Abcam community

    Verified customer

    Asked on Feb 27 2013

    Answer

    Your protocol appears to be correct. The only IHC data we have is from customers who used citrate heat-mediated retrieval and dilutions of 1/1000 or 1/800. The samples were hypertrophic scar and a subcutaneous implant of MDA-MB 436 breast cancer cells. Are you confident that your secondary antibody is effective? It should be anti-rabbit IgG. The SFRP1 protein is located in the plasma membrane but it is also secreted, which may account for staining in the extra-cellular matrix.

    Here are links to the reviews:

    https://www.abcam.com/SFRP1-antibody-ab4193-reviews.html?intabreviewid=15717

    https://www.abcam.com/SFRP1-antibody-ab4193-reviews.html?intabreviewid=9877

    Read More

    Abcam Scientific Support

    Answered on Feb 27 2013

    Question

    I am trying to standardise SFRP 1 antibody (ab4193) 1. Could u please suggest a good positive control for membranous staining for using this antibody for immunohistochemistry?? 2. What type of antigen retrieval method will be best suited for this antibody? I'll be highly obliged .

    Read More

    Abcam community

    Verified customer

    Asked on Feb 13 2013

    Answer

    The highest expression is in the heart and kidney. For an example of a strong stain of heart muscle tissue, please see the image on the datasheet for a different SFRP1 antibody, ab126613:

    Click here (or use the following: https://www.abcam.com/SFRP1-antibody-EPR7003-ab126613.html).

    For ab4193, we recommend a heat-mediated antigen retrieval, based on the customer review from 2008:

    https://www.abcam.com/SFRP1-antibody-ab4193-reviews.html?intabreviewid=9877

    The reviewer used a DAKO retrieval solution but a 10mM sodium citrate buffer, pH 6.0, should be sufficient.

    Read More

    Abcam Scientific Support

    Answered on Feb 13 2013

    Question

    Please see below for email we have received from a customer;

    I have filled up the form and included the images from the staining that finished this morning and one image from previous experiment to you. We have purchased 2 tube of this antibody from Abcam previously (lot#GR11572-4), it worked excellent. We don’t know why this lot of the antibody didn’t work well. We hope you are able to solve this problem, that we can get the good result from this study.

    Many thanks for your time on this issue!

    Sapphire Order #: PO-15778

    Abcam Invoice #: 1354924A

    Thanks for your help and hope to hear from you soo

    Kind regards,

    Read More

    Abcam community

    Verified customer

    Asked on Nov 13 2012

    Answer

    Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.

    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

    Read More

    Abcam Scientific Support

    Answered on Nov 13 2012

    Question

    Good morning

    I am enclosing the questionnaire answers and images you asked me.
    Thanks.
    Best regards.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 25 2012

    Answer

    Thank you very much for providing that information. It has been very helpful in understanding the results you have seen so far in your experiment.

    I am sorry but Kate is currently away from the office but I will try my best to help you with these problems.

    It is clear that you have put a lot of effort in to try and optimise the results with these antibodies. It is regrettable that the results so far have not been as hoped. Would you mind clarifying a few further details for me in order to more fully understand what may be contributing to this?

    1. You have mentioned that you have purchased three antibodies from us. Is the third one working as expected? Which antibody was this?

    2. Have you been performing any staining of the tissues with any other antibodies? How has this staining performed?

    3. How were the tissues fixed (time and temperature in formalin)?

    4. Can I ask, the antigen retrieval, am I correct in thinking you tried the three different buffers individually, each for 20 minutes at 97oC? How was this performed, in a microwave or pressure cooker etc? You also mentioned it being performed for 30 minutes, 1 hour, 2 hours and overnight, could you clarify this?

    Once I have this information I hope to be able to understand more fully what may be contributing to the staining observed currently.

    look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    Answered on Oct 25 2012

    Question

    Thanks for your attention.

    I am using human breast carcinoma tissue as positive control for NPR3 antibody and skin (keratinocytes) as positive control for SFRP1 antibody (like descripted in your datasheet). I started my reactions with 1:200 concentration for NPR3 and 1:2000 for SFRP1, as suggested by Michael Taylor, MD in his paper "Medulloblastoma comprises four distinct molecular variants".

    However, after five differents attempts (over night, 60 minutes and 1 hour of incubation, with Ultra Vision Plus detection (Thermo)), I think this protocols worked better: 1:50 concentration for NPR3 with 2 hours of incubation, pH 6,0, antigen retrieval in PT-Link (DAKO) and DAKO detection.

    In case of SFRP1, I did the same protocols above with up 1:500 in concentration and I didn't get any staining.

    Please find the best images attached.

    Best regards.


    2012/10/12 <mailto:technical@abcam.com>














    https://www.abcam.com/index.html?utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Header








    Dear Gisele



    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibody.

    I would like to reassure you that our antibodies are tested and covered by our 6 month guarantee for the tested species and applications listed on the individual datasheets. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would appreciate if you could let me know which application you have been using. Also which type of samples you have tested. Once I know which application you are using, I can obtain the protocol from the laboratory and will be pleased to send this on to you.

    I would also appreciate if you are able to provide an image which would help us to assess the results.

    Thank you for your time and cooperation. I look forward to hearing from you with the requested information.

    Help us improve our service.
    https://www.abcam.com/index.html?pageConfig=technicalSurvey&intCCEID=4242373


    Best regards,
    Kate

    Kate Hayes
    Scientific Support Supervisor
    Abcam plc
    https://www.abcam.com/


    Your original inquiry to Abcam:














    From: Gisele Caravina de Almeida [mailto:giselecaravina@gmail.com]
    Sent: 11 October 2012 16:22
    To: Licensing; Careers; Webmaster; Gisele Caravina de Almeida
    Subject: I need help about my reactions to SFRP1 and NPR3









    Hello,







    My name is Gisele and I bought three antibodies of Abcam but two of them aren´t working: SFRP1 (ab4193) and NPR3 (ab37617). I did several protocols but I haven´t got satisfactory result.



    I would like to get your protocols for these antibodies to repeat my reactions.



    Thanks.










    Abcam Customer Services and Scientific Support Team
    https://www.abcam.com/index.html?pageconfig=technical&utm_campaign=CRM&utm_source=Abcam.CRM&utm_medium=Email&utm_term=Body

    [CCE4242373]


















    Discover more at http://abcam.com/

    Read More

    Abcam community

    Verified customer

    Asked on Oct 16 2012

    Answer

    Thank you for taking the time to reply with the information.

    The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

    I would like to reassure you that ab4193 is tested and covered by our 6 month guarantee for use in IHC-P and human samples. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

    Although the other antibody has worked well using this procedure, individual antibodies will often require optimization. Before deciding how to proceed, I would like to investigate this particular case further for you, and also obtain some further information for our quality records.

    In order to do this, I have enclosed a questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. I have completed the sections for which information has already been provided.

    In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    Thank you for your time and cooperation. We look forward to receiving the completed questionaire.



    Order Details
    Antibody code:

    Lot number:


    Purchase order number
    or preferably Abcam order number:


    General Information
    Antibody storage conditions (temperature/reconstitution etc)


    Description of the problem (high background, low signal, non-specific staining etc.)

    Sample (Species/Tissue/Cell Type/Cell Line etc.)

    breast carcinoma tissue, skin (keratinocytes)

    Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)


    Antigen retrieval (Enzymatic method, Heat mediated technique etc.)

    pH 6,0, antigen retrieval in PT-Link (DAKO)

    What different incubation times and buffers been tried for antigen retreival to optimize?


    Permeabilization step


    Blocking conditions (Buffer/time period, Blocking agent etc.)


    Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    1:500 (over night, 60 minutes and 1 hour of incubation)

    Please confirm what other dilutions have been tried.

    Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)


    Is the current vial of secondary antibody working well with other primary antibodies?

    Detection method


    Positive and negative controls used (please specify)


    Optimization attempts (problem solving)
    How many times have you tried the IHC?



    Have you run a "No Primary" control?
    Yes No

    Do you obtain the same results every time?
    Yes No


    What steps have you altered?


    Additional Notes


    We would appreciate if you are also able to provide and image which woudl help us to assess the results

    Read More

    Abcam Scientific Support

    Answered on Oct 16 2012

    Question

    Hello,

    I bought three antibodies of Abcam but two of them aren't working: SFRP1 (ab4193) and NPR3 (ab37617). I did several protocols but I haven´t got satisfactory result.

    I would like to get your protocols for these antibodies to repeat my reactions.

    Thanks.

    Read More

    Abcam community

    Verified customer

    Asked on Oct 12 2012

    Answer

    Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibody.

    I would like to reassure you that our antibodies are tested and covered by our 6 month guarantee for the tested species and applications listed on the individual datasheets. In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

    I would like to investigate this particular case further for you, and also obtain more information for our quality monitoring records. In order to proceed with this, I would appreciate if you could let me know which application you have been using. Also which type of samples you have tested. Once I know which application you are using, I can obtain the protocol from the laboratory and will be pleased to send this on to you.

    I would also appreciate if you are able to provide an image which would help us to assess the results.

    Thank you for your time and cooperation. I look forward to hearing from you with the requested information.

    Read More

    Abcam Scientific Support

    Answered on Oct 12 2012

    Question

    Buenos días , ruego nos envíes el reemplazo del anticuerpo, de uno de los lotes que tenéis disponibles.
    Para que no tengamos problemas en la entrega, sería mejor que figure en el albarán, el pedido inicial.
    Muchas gracias.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 05 2012

    Answer

    Este es un mail de confirmación del nuevo pedido, consistente en el reemplazo completamente gratuito, del anticuerpo ab4193 con número de pedido XXX.

    Os llegará un mail de confirmación del nuevo pedido, si necesitáis información sobre el estado del mismo no dudéis en contactarnos haciendo referencia al número indicado.

    Lamento mucho las molestias ocasionadas. No dudéis en contactarnos para cualquier otra duda o información.

    Read More

    Abcam Scientific Support

    Answered on Sep 05 2012

    Question

    Buenos días, adjunto envío la RECLAMACIÓN de un anticuerpo que no ha funcionado.
    Espero su respuesta.

    Saludos,

    Read More

    Abcam community

    Verified customer

    Asked on Sep 03 2012

    Answer

    Gracias por contactarnos y alertarnos del problema sufrido con este anticuerpo.

    Efectivamente los resultados obtenidos con este nuevo lote no se corresponden con los esperados, y le pido disculpas por las molestias que esto haya podido ocasionar.

    Dado que nuestros productos tienen 6 meses de garantía en caso de no funcionar como se especifica en su datasheet, os reemplazaré el producto por un lote diferente. Sin embargo, no tenemos actualmente en stock el lote que os dió buenos resultados en el pasado.

    Tenemos otros que difieren de los dos ya testados, si me confirmáis que queréis recibir el reemplazo por uno de ellos, crearé el pedido de inmediato. Alternativamente podemos sustituir el anticuerpo por otro diferente de nuestro catálogo, o reembolsaros el importe.

    Quedo a la espera de recibir su respuesta para proceder.

    Read More

    Abcam Scientific Support

    Answered on Sep 03 2012

    ELISA abreview for Anti-SFRP1 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    ELISA
    Sample
    Human Purified protein (Tears)
    Specification
    Tears
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Type
    Sandwich (Detection)
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 24 2012

    Question

    Thank you for your email.
    The link for the article is
    http://www.ncbi.nlm.nih.gov/pubmed/20823417
    COuld you please let me know which antibody products they
    used against antigens SFRP1, NPR3, KCNA1?
    Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Aug 10 2012

    Answer

    Thank you for your email.

    I apologize for the delay as it took a while to get back to you. Unfortunately the publication team at Abcam is unable to trace back the antibodies catalogue number the author have used. They can only trace one abID which is ab4193.

    I would suggest contacting the author for this information. I am sure they will be able to release this information.

    I hope this information is nevertheless helpful. Should you have any further question please do not hesitate to contact me.

    Read More

    Abcam Scientific Support

    Answered on Aug 10 2012

    1-10 of 19 Abreviews or Q&A

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